Antonie van Leeuwenhoek

, Volume 101, Issue 4, pp 819–835 | Cite as

Spatial and temporal analysis of estuarine bacterioneuston and bacterioplankton using culture-dependent and culture-independent methodologies

  • Juliana S. N. Azevedo
  • Isabel Ramos
  • Susana Araújo
  • Cláudia S. Oliveira
  • António Correia
  • Isabel S. Henriques
Original Paper

Abstract

Bacterioneuston may play a key role in water–air exchange of gases and in processing organic matter and pollutants that accumulate at the sea-surface microlayer (SML). However, the phylogenetic diversity of bacterioneuston has been poorly characterized. We analyzed 24 samples each from the SML and underlying water (UW) at three sites in the Ria de Aveiro estuary, Portugal. Cultivation and culture-independent techniques were used to compare bacterioneuston and bacterioplankton. Culturable heterotrophic bacteria were enriched in the SML. The culturable community was dominated by Psychrobacter and Acinetobacter. The presence of high numbers of Psychrobacter was a notable result. Differences were confined to a few genera overrepresented in UW samples (Kocuria, Agrococcus and Vibrio). 16S rDNA DGGE profiles were highly stable in terms of number and position of bands between sampling sites and dates but cluster analysis revealed a slight tendency for grouping according to sampled layer. SML-specific DGGE bands affiliated with Actinobacteria, Cyanobacteria, Gammaproteobacteria and Bacteroidetes. Low similarity between nucleotide sequences of DGGE-bands and previously reported sequences suggest the occurrence of SML-specific populations. Enrichment of SML for Pseudomonas and Aeromonas was questioned and the diversity of both communities was analyzed. Consistent differences between SML and UW aeromonads communities were not identified. In terms of Pseudomonas, a culturable operational taxonomic unit was consistently overrepresented within SML samples. Taken together, our results indicate that the similarity between SML and UW communities depends on spatial and temporal factors.

Keywords

Phylogenetic diversity biofilm Sea-surface microlayer Estuary DGGE 

Notes

Acknowledgments

This work was financed by the Fundacão para a Ciência e a Tecnologia (FCT) through grants SFRH/BPD/65820/2009 (CSO), SFRH/BD/64057/2009 (JSNA) and SFRH/BPD/21384/2005 (IH) and by the European Union Program of High Level Scholarships for Latin America––Program Alβan––through grant E07D403901BR (JSNA). The authors wish to thank Ângela Cunha and Adelaide Almeida for technical support (sampling device, conductivity meter, etc.). We are also grateful to A. Moura (Department of Biology, University of Aveiro) for reviewing English style and grammar.

Supplementary material

10482_2012_9697_MOESM1_ESM.doc (320 kb)
Supplementary material 1 (DOC 320 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Juliana S. N. Azevedo
    • 1
  • Isabel Ramos
    • 2
    • 3
  • Susana Araújo
    • 2
  • Cláudia S. Oliveira
    • 1
  • António Correia
    • 1
  • Isabel S. Henriques
    • 1
  1. 1.CESAM and Department of BiologyUniversity of AveiroAveiroPortugal
  2. 2.Department of BiologyUniversity of AveiroAveiroPortugal
  3. 3.Department of Microbiology, Faculty of MedicineUniversity of PortoPortoPortugal

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