Antonie van Leeuwenhoek

, Volume 101, Issue 1, pp 147–154

Ornithinibacillus scapharcae sp. nov., isolated from a dead ark clam

  • Na-Ri Shin
  • Tae Woong Whon
  • Min-Soo Kim
  • Seong Woon Roh
  • Mi-Ja Jung
  • Young-Ok Kim
  • Jin-Woo Bae
Original Paper

DOI: 10.1007/s10482-011-9645-3

Cite this article as:
Shin, NR., Whon, T.W., Kim, MS. et al. Antonie van Leeuwenhoek (2012) 101: 147. doi:10.1007/s10482-011-9645-3

Abstract

A novel Gram-positive, aerobic, motile, hemolytic, endospore-forming and rod-shaped bacterium TW25T was isolated from a dead ark clam during a mass mortality event on the South coast of Korea. The strain grew optimally at 30°C, at pH 8–9, and with 1% (w/v) NaCl. The 16S rRNA gene sequence analysis indicated that strain TW25T was associated with the genus Ornithinibacillus and that it was most closely related to the type strain of Ornithinibacillus californiensis (98.5% similarity). The dominant cellular fatty acids were iso-C15:0, anteiso-C15:0 and C16:0. The peptidoglycan amino acid type was A4β, containing l-ornithine and d-aspartic acid. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids, two unidentified aminolipids and two unidentified lipids. The major respiratory quinone was menaquinone-7 (MK-7). The G + C content of genomic DNA was 36.7 mol%. DNA–DNA hybridization experiments with related strains revealed lower than 11 ± 3% relatedness. Based on this polyphasic taxonomic study, strain TW25T represents a novel species in the genus Ornithinibacillus, for which the name Ornithinibacillus scapharcae sp. nov. is proposed. The type strain is TW25T (=KACC 15116T = JCM 17314T).

Keywords

Ornithinibacillus scapharacae Polyphasic taxonomy Ark clam A4β type peptidoglycan 

Supplementary material

10482_2011_9645_MOESM1_ESM.ppt (2.9 mb)
Supplementary Fig. S1. Phase contrast micrograph of strain TW25T after growth on TSA for 24 h, showing the endospore with swollen sporangia (A), and transmission electron micrograph of a cell of strain TW25T (B). Arrow indicates the peritrichous flagella of strain TW25T. (PPT 2995 kb)
10482_2011_9645_MOESM2_ESM.ppt (240 kb)
Supplementary Fig. S2. Two-dimensional thin-layer chromatograms of polar lipids from strain TW25T. Total lipids were revealed using Ornithinibacillus bavariensis WSBC 24001T. Abbreviations: DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PL1-4, unidentified phospholipids; AL1-2, unidentified aminolipids; L1-2, unidentified lipids. (PPT 239 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Na-Ri Shin
    • 1
  • Tae Woong Whon
    • 1
  • Min-Soo Kim
    • 1
  • Seong Woon Roh
    • 1
  • Mi-Ja Jung
    • 1
  • Young-Ok Kim
    • 2
  • Jin-Woo Bae
    • 1
  1. 1.Department of Life and Nanopharmaceutical Sciences and Department of BiologyKyung Hee UniversitySeoulKorea
  2. 2.Biotechnology Research DivisionNational Fisheries Research and Development InstituteGijang, BusanRepublic of Korea

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