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Antonie van Leeuwenhoek

, Volume 101, Issue 2, pp 303–311 | Cite as

Oxygen exposure increases resistance of Desulfovibrio vulgaris Hildenborough to killing by hydrogen peroxide

  • Janine D. Wildschut
  • Sean M. Caffrey
  • Johanna K. Voordouw
  • Gerrit Voordouw
Original Paper

Abstract

Inactivation of PerR by oxidative stress and a corresponding increase in expression of the perR regulon genes is part of the oxidative stress defense in a variety of anaerobic bacteria. Diluted anaerobic, nearly sulfide-free cultures of mutant and wild-type Desulfovibrio vulgaris (105–106 colony-forming units/ml) were treated with 0 to 2,500 μM H2O2 for only 5 min to prevent readjustment of gene expression. Survivors were then scored by plating. The wild type and perR mutant had 50% survival at 58 and 269 μM H2O2, respectively, indicating the latter to be 4.6-fold more resistant to killing by H2O2 under these conditions. Significantly increased resistance of the wild type (38-fold; 50% killing at 2188 μM H2O2) was observed if cells were pretreated with full air for 30 min, conditions that did not affect cell viability. The resistance of the perR mutant increased less (4.6-fold; 50% killing at 1230 μM H2O2), when similarly pretreated. Interestingly, no increased resistance of either was achieved by exposure with 10.6 μM H2O2 for 30 min, the highest concentration that could be used without killing the cells. Hence, in environments with low D. vulgaris biomass only the presence of external O2 effectively activates the perR regulon. As a result, mutant strains lacking one of the perR regulon genes ahpC, dvu0772, rbr1 or rbr2 displayed decreased resistance to H2O2 stress only following pretreatment with air.

Keywords

Sulfate-reducing bacteria Hydrogen peroxide Oxidative stress PerR Rubrerythrin Desulfovibrio 

Abbreviations

SRB

Sulfate-reducing bacteria

D

Desulfovibrio

OD

Optical density

CFU

Colony forming units

PCR

Polymerase chain reaction

Notes

Acknowledgment

This work was supported by an NSERC Discovery Grant to GV. GV also acknowledges salary support from an NSERC Industrial Research Chair Award.

Supplementary material

10482_2011_9634_MOESM1_ESM.doc (281 kb)
Fig. S1. Immunoblot analysis of Rbr2 content in D. vulgaris strains. (DOC 281 kb)
10482_2011_9634_MOESM2_ESM.doc (56 kb)
Table S1. Primers used for replacement mutagenesis in this study (DOC 56 kb)
10482_2011_9634_MOESM3_ESM.doc (54 kb)
Table S2. Genes with significantly changed expression ratio’s for perR mutant versus wild type. (DOC 53 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Janine D. Wildschut
    • 1
  • Sean M. Caffrey
    • 2
  • Johanna K. Voordouw
    • 2
  • Gerrit Voordouw
    • 2
  1. 1.Summit Liability Solutions Inc.CalgaryCanada
  2. 2.Petroleum Microbiology Research Group, Department of Biological SciencesUniversity of CalgaryCalgaryCanada

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