Antonie van Leeuwenhoek

, 100:537 | Cite as

Streptomyces fildesensis sp. nov., a novel streptomycete isolated from Antarctic soil

Original Paper
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Abstract

A novel actinomycete strain, GW25-5T, was isolated from a soil sample collected from the Fildes Peninsula, King George Island, West Antarctica. The strain was characterized by white to grey aerial mycelia, which were differentiated to straight to flexuous spore chains, with rod-shaped smooth spores. The cell wall of strain GW25-5T contained LL-diaminopimelic acid (A2pm) and traces of meso-A2pm. Whole-cell sugars were galactose and minor amounts of mannose and glucose. The predominant menaquinones were MK-9(H6) (49%), MK-9(H8) (24%) and MK-9(H4) (12%). The phospholipids contained DPG, PE, PI, PIM and PL(s). The major cellular fatty acids were iso-C16:0 and anteiso-C15:0. Genomic DNA G+C content of strain GW25-5T was 70.0 mol%. BLAST result showed that strain GW25-5 has the 16S rRNA gene sequence highest similarity of 97.5% with members of genus Streptomyces and phylogenetic analysis indicated that this strain belongs to the genus Streptomyces. DNA–DNA relatedness values of strain GW25-5T with the closest species of Streptomyces purpureus LMG 19368T and Streptomyces beijiangensis YIM 6T were significantly lower than 70% of the threshold value for the delineation of genomic species. A polyphasic taxonomic investigation based on a judicious combination of genotypic and phenotypic characteristics revealed that the organism represents a novel species of the genus Streptomyces. Thus, we propose strain GW25-5T as the type strain of this novel species, Streptomyces fildesensis (=CGMCC 4.5735T = YIM 93602T = DSM 41987T = NRRL B 24828T).

Keywords

Streptomyces fildesensis sp. nov. 16S rRNA Antarctic 
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Notes

Acknowledgments

This research was supported by the National Basic Research Program of China (No. 2010CB833801), The National Natural Science Foundation of China (40906075, 40906076), Shandong Provincial Science and Technology Development Program (project no.2009GG10002082), State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (SKLMR-080601) and the SOA Key Laboratory for Polar Science (KP2005013). The authors acknowledge the support of Chinese Arctic and Antarctic Administration of the State Oceanic Administration.

Supplementary material

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Supplementary material 1 (DOC 1357 kb)

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© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.College of Marine Life SciencesOcean University of ChinaQingdaoPeople’s Republic of China
  2. 2.Key Laboratory of Marine Bio-resources Sustainable Utilization, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; South China Sea Institute of OceanologyChinese Academy SciencesGuangzhouPeople’s Republic of China
  3. 3.Key Laboratory of Marine Drugs of the Ministry of Education, School of Medicine and PharmacyOcean University of ChinaQingdaoPeople’s Republic of China
  4. 4.The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of MicrobiologyYunnan UniversityKunmingPeople’s Republic of China

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