Antonie van Leeuwenhoek

, Volume 100, Issue 3, pp 477–481 | Cite as

Bacteria associated with the pinewood nematode Bursaphelenchus xylophilus collected in Portugal

  • Cláudia S. L. Vicente
  • Francisco Nascimento
  • Margarida Espada
  • Manuel Mota
  • Solange OliveiraEmail author
Short Communication


In this study, we report on the bacterial community associated with the pinewood nematode Bursaphelenchus xylophilus from symptomatic pine wilted trees, as well as from long-term preserved B. xylophilus laboratory collection specimens, emphasizing the close bacteria–nematode associations that may contribute to pine wilt disease development.


Pine wilt disease Bursaphelenchus xylophilus Nematode Bacteria Phylogeny 



This work was supported by the Portuguese Project Programa de Acção Nacional para Controlo do Nemátodo da Madeira do Pinheiro: “A doença do nemátode da madeira do pinheiro Bursaphelenchus xylophilus” from Instituto de Financiamento da Agricultura e Pescas (IFAP) and Autoridade Florestal Nacional (AFN). The authors wish to thank Isabel Abrantes, Paula Morais and their research team from University of Coimbra (Coimbra, Portugal), for the support given in the sampling collection.


  1. Ashelford KE, Chuzhanova NA, Fry JC, Jones AJ, Weightman AJ (2005) At least 1 in 20 16S rRNA sequence records currently held in public repositories is estimated to contain substantial anomalies. Appl Environ Microbiol 71:7724–7736PubMedCrossRefGoogle Scholar
  2. Bai Y, Souleimanov A, Smith DL (2002) An inducible activator produced by a Serratia proteamaculans strain and its soybean growth-promoting activity under greenhouse conditions. J Exp Bot 53:1495–1502PubMedCrossRefGoogle Scholar
  3. Barash I, Manulis-Sasson S (2009) Recent evolution of bacterial pathogens: the gall-forming Pantoea agglomerans case. Annu Rev Phytopathol 47:133–152PubMedCrossRefGoogle Scholar
  4. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  5. Han ZM, Hong YD, Zhao BG (2003) A study on pathogenicity of bacteria carried by pine wood nematodes. J Phytopathol 151:683–689CrossRefGoogle Scholar
  6. Hongoh Y, Yuzawa H, Ohkuma M, Kudo T (2003) Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment. FEMS Microbiol Lett 221:299–304PubMedCrossRefGoogle Scholar
  7. Kawazu K, Zhang H, Kanzaki H (1996a) Accumulation of benzoic acid in suspension cultured cells of Pinus thunbergii Parl. in response to phenylacetic acid administration. Biosci Biotechnol Biochem 60:1410–1412PubMedCrossRefGoogle Scholar
  8. Kawazu K, Zhang H, Yamashita H, Kanzaki H (1996b) Relationship between the pathogenicity of the pine wood nematode, Bursaphelenchus xylophilus, and phenylacetic acid production. Biosci Biotechnol Biochem 60:1413–1415PubMedCrossRefGoogle Scholar
  9. Kiyohara T, Tokushige Y (1971) Inoculation experiments of a nematode, Bursaphelenchus sp., onto pine trees. J Jpn For Sci 53:210–218Google Scholar
  10. Kwon H, Choi G, Choi Y, Jang K, Sung N, Kang M, Moon Y, Lee S, Kim J (2010) Suppression of pine wilt disease by an antibacterial agent, oxolinic acid. Pest Manag Sci 66:634–639PubMedGoogle Scholar
  11. Laranjo M, Machado J, Young J, Oliveira S (2004) High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region. FEMS Microbiol Ecol 48:101–107PubMedCrossRefGoogle Scholar
  12. Li H (2007) Identification and pathogenicity of Bursaphelenchus species (Nematoda: Parasitaphelenchidae). Ph.D. thesis, University of GhentGoogle Scholar
  13. Mohamed ZK, El-wahab GS (2009) Potency evaluation of Serratia marcescens and Pseudomonas fluorescens as biocontrol agents for root-knot nematodes in Egypt. J Appl Sci Res 4:93–102Google Scholar
  14. Mota M, Braasch H, Bravo M, Penas A, Burgermeister W, Metge K, Sousa E (1999) First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1:727–734CrossRefGoogle Scholar
  15. Oku H, Shiraishi T, Kurozumi S (1980) Pine wilt toxin, the metabolite of a bacterium associated with a nematode. Naturwissenschaften 67:198–199CrossRefGoogle Scholar
  16. Proença DN, Francisco R, Santos CV, Lopes A, Fonseca L, Abrantes IM, Morais PV (2010) Diversity of bacteria associated with Bursaphelenchus xylophilus and other nematodes isolated from Pinus pinaster trees with pine wilt disease. PLoS ONE 5:e15191PubMedCrossRefGoogle Scholar
  17. Rodrigues J (2008) National eradication programme for pinewood nematode. In: Vieira P, Mota M (eds) Pine wilt disease: a worldwide threat to forest ecosystems. Springer, Dordrecht, pp 5–14CrossRefGoogle Scholar
  18. Staden R (1996) The Staden sequence analysis package. Mol Biotechnol 5:233–241PubMedCrossRefGoogle Scholar
  19. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599PubMedCrossRefGoogle Scholar
  20. Xie B, Cheng X, Shi J, Zhang Q, Dai S, Cheng F, Luo Y (2009) Mechanisms of invasive population establishment and spread of pinewood nematodes in China. Sci China C 52:587–594CrossRefGoogle Scholar
  21. Zhao B, Lin F (2005) Mutualistic symbiosis between Bursaphelenchus xylophilus and bacteria of the genus Pseudomonas. For Pathol 35:339–345CrossRefGoogle Scholar
  22. Zhao B, Ang H, An S, An Z (2003) Distribution and pathogenicity of bacteria species carried by Bursaphelenchus xylophilus in China. Nematology 5:899–906CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Cláudia S. L. Vicente
    • 1
  • Francisco Nascimento
    • 1
  • Margarida Espada
    • 1
  • Manuel Mota
    • 1
  • Solange Oliveira
    • 2
    Email author
  1. 1.NemaLab, Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM)Universidade de ÉvoraÉvoraPortugal
  2. 2.Laboratório de Microbiologia do Solo, Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM)Universidade de ÉvoraÉvoraPortugal

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