Antonie van Leeuwenhoek

, Volume 100, Issue 3, pp 333–339 | Cite as

Amycolatopsis endophytica sp. nov., a novel endophytic actinomycete isolated from oil-seed plant Jatropha curcas L.

  • Qian Miao
  • Sheng Qin
  • Guang-Kai Bian
  • Bo Yuan
  • Ke Xing
  • Yue-Ji Zhang
  • Qin Li
  • Shu-Kun Tang
  • Wen-Jun Li
  • Ji-Hong Jiang
Original Paper

Abstract

A novel actinomycete, designated KLBMP 1221T, was isolated from the surface-sterilized seeds of an oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China and was characterized taxonomically by using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequence showed that this strain formed a distinct phyletic line within the radiation of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1221T was most closely related to Amycolatopsis eurytherma NT202T (98.9%), Amycolatopsis tucumanensis ABOT (98.8%), Amycolatopsis thermoflava N1165T (98.6%) and Amycolatopsis methanolica IMSNU 20055T (98.5%). Strain KLBMP 1221T had morphological and chemotaxonomic properties that were consistent with its classification in the genus Amycolatopsis. However, DNA–DNA relatedness data and phenotypic differences clearly distinguished the isolate from its closest relatives. Based on the combined genotypic and phenotypic evidence, it is proposed that strain KLBMP 1221T be classified as representative of a novel species for which the name Amycolatopsisendophytica sp. nov. is proposed. The type strain is KLBMP 1221T (= KCTC 19776T = CCTCC AA 2010003T).

Keywords

Amycolatopsis endophytica sp. nov. Polyphasic taxonomy 16S rRNA 

Supplementary material

10482_2011_9588_MOESM1_ESM.docx (188 kb)
Supplementary material 1 (DOCX 187 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Qian Miao
    • 1
  • Sheng Qin
    • 1
  • Guang-Kai Bian
    • 1
  • Bo Yuan
    • 1
  • Ke Xing
    • 1
  • Yue-Ji Zhang
    • 1
  • Qin Li
    • 1
  • Shu-Kun Tang
    • 2
  • Wen-Jun Li
    • 2
  • Ji-Hong Jiang
    • 1
  1. 1.School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu ProvinceXuzhou Normal UniversityXuzhouPeople’s Republic of China
  2. 2.The Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of MicrobiologyYunnan UniversityKunmingPeople’s Republic of China

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