Antonie van Leeuwenhoek

, Volume 99, Issue 4, pp 845–854 | Cite as

Rhizobium sphaerophysae sp. nov., a novel species isolated from root nodules of Sphaerophysa salsula in China

  • Lin Xu
  • Jian Feng Shi
  • Peng Zhao
  • Wei Min Chen
  • Wei Qin
  • Ming Tang
  • Ge Hong Wei
Original Paper


Four gram-negative, aerobic, motile, non-spore, forming rods with a wide pH and temperature range for growth (pH 7.0–11.0, optimum pH 8.0; 20–45°C, optimum 28°C) strains were isolated from root nodules of Sphaerophysa salsula and characterized by means of a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the four strains formed a new lineage related to the genus Rhizobium and the sequence similarities between the isolate and the most related type strain Rhizobium giardinii was 96.5%. These strains also formed a distinctive group from the reference strains for defined Rhizobium species based on housekeeping gene sequences (atpD and recA), BOX-PCR fingerprinting, phenotypic features and symbiotic properties. The representative strain CCNWGS0238T has DNA-DNA relatedness of less than 33.4% with the most closely related species R. giardinii. It is therefore proposed as a new species, Rhizobium sphaerophysae sp. nov., with isolate CCNWGS0238T (=ACCC17498T = HAMBI3074T) as the type strain.


Sphaerophysa salsula Rhizobia Taxonomy 



This work was supported by projects from the 973 Project of China (2010CB126502), National Science Foundation of China (30970003, 30900215 and 30630054), International Science & Technology Cooperation Program of China (2010DFA91930). We are grateful to Dr. Kristina Lindström and Prof. Ruibo Jiang for deposition of the strain in the culture collections.

Supplementary material

10482_2011_9559_MOESM1_ESM.ppt (68 kb)
Supplementary material 1 (PPT 67 kb)
10482_2011_9559_MOESM2_ESM.ppt (83 kb)
Supplementary material 2 (PPT 83 kb)


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Lin Xu
    • 1
    • 2
  • Jian Feng Shi
    • 2
  • Peng Zhao
    • 1
  • Wei Min Chen
    • 1
  • Wei Qin
    • 1
  • Ming Tang
    • 1
  • Ge Hong Wei
    • 1
  1. 1.College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for AgricultureNorthwest A & F UniversityYangling ShaanxiChina
  2. 2.Department of Life Science and EngineeringHexi UniversityZhangye GansuChina

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