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Antonie van Leeuwenhoek

, Volume 99, Issue 3, pp 721–726 | Cite as

Description of a novel actinobacterium Kocuria assamensis sp. nov., isolated from a water sample collected from the river Brahmaputra, Assam, India

  • Chandandeep Kaur
  • Ishwinder Kaur
  • Revti Raichand
  • Tarun Chandra Bora
  • Shanmugam Mayilraj
Original Paper

Abstract

A Gram-positive, pale yellow pigmented actinobacterium, strain S9-65T was isolated from a water sample collected from the river Brahmaputra, Assam, India and subjected to a polyphasic taxonomic study. The physiological and biochemical properties, major fatty acids (anteiso-C15:0 and anteiso-C17:0), estimated DNA G+C content (69.2 mol %) and 16S rRNA gene sequence analysis showed that strain S9-65T belonged to the genus Kocuria. Strain S9-65T exhibited highest 16S rRNA gene sequence similarity with Kocuria palustris (99.1%); however, the DNA–DNA relatedness value between strain S9-65T and K. palustris was 20.6%. On the basis of differential phenotypic characteristics and genotypic distinctiveness, strain S9-65T should be classified as representative of a novel species Kocuria, for which the name Kocuria assamensis is proposed. The type strain is S9-65T (=MTCC 10622T = DSM 23999T).

Keywords

DNA–DNA hybridization FAME 16S rRNA gene sequence 

Notes

Acknowledgments

We thank Mr Malkit Singh for his excellent technical assistance. This work was supported by CSIR (net work project NWP006) Government of India. This is IMTECH communication number 073/2010.

Supplementary material

10482_2010_9547_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1332 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Chandandeep Kaur
    • 1
  • Ishwinder Kaur
    • 1
  • Revti Raichand
    • 1
  • Tarun Chandra Bora
    • 2
  • Shanmugam Mayilraj
    • 1
  1. 1.Microbial Type Culture Collection (MTCC) & Gene BankInstitute of Microbial TechnologyChandigarhIndia
  2. 2.Department of BiotechnologyNorth East Institute of Science & TechnologyJorhatIndia

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