Antonie van Leeuwenhoek

, Volume 99, Issue 3, pp 663–670 | Cite as

Halomonas aidingensis sp. nov., a moderately halophilic bacterium isolated from Aiding salt lake in Xinjiang, China

  • Wen-Yan LiuEmail author
  • Juan WangEmail author
  • Meng Yuan
Original Paper


Two Gram-negative moderately halophilic bacterial strains, designated Ad-1T and C-12, were isolated from Aiding salt lake of Xinjiang in China. The novel isolates were subjected to a polyphasic taxonomic study. Cells of these strains were cocci or short rods and motile with polar flagella. Colonies produced brown-red pigment. The isolates grew in the range of 0.5–25% (w/v) NaCl, pH 5.5–10.5 and 4–45°C. Analysis of their 16S rRNA gene sequences indicated that strains Ad-1T and C-12 belonged to the genus Halomonas showing 92.7–98.4% similarity with the type species. The isoprenoid quinones of the isolates were Q-9 and Q-8. The major cellular fatty acids were C18:1ω7c, C16:1ω7c/6c, C16:0, C12:0–3OH and C10:0. The DNA G + C contents of strains Ad-1T and C-12 were 64.6 and 63.9 mol%, respectively. The DNA relatedness between the two isolates was 89.2%. The similarities of these newly isolated strains with closely related type strains were lower than 35% at the genetic level. Based on phenotypic, chemotaxonomic and genetic characteristics, the representative strain Ad-1T is considered to be a novel species of the genus Halomonas, for which the name Halomonas aidingensis sp. nov. is proposed, with Ad-1T (= CGMCC 1.10191T = NBRC 106173T) as the type strain.


Halomonas aidingensis sp. nov. Moderate halophile Salt lake Gram-negative 



This work was supported financially by the Program for New Century Excellent Talents in Xiamen University to Prof. Chun Fan. We are grateful to Yuguang Zhou (China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences), for helping in microbiological culture collection.

Supplementary material

10482_2010_9542_MOESM1_ESM.pdf (1 mb)
Supplementary Figure S1 Supplementary Figure S1. Transmission electron micrograph of strain Ad-1T. Bar, 1 μm Supplementary Figure S2 (a & b) Supplementary Figure S2. Phylogenetic tree based on 16S rRNA gene sequences. Numbers at branching points refer to bootstrap values (1000 resamplings; only values above 50% are shown). (a) minimum-evolution tree. (b) maximum-parsimony tree. (PDF 1047 kb)


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.National Engineering Laboratory of BiohydrometallurgyGeneral Research Institute for Nonferrous MetalsBeijingPeople’s Republic of China
  2. 2.Department of Preventive MedicineMedical College of Xiamen UniversityXiamenPeople’s Republic of China
  3. 3.Nanshan District Center for Disease Control and PreventionShenzhen, GuangdongPeople’s Republic of China

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