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Antonie van Leeuwenhoek

, Volume 99, Issue 3, pp 629–634 | Cite as

Methylobacterium soli sp. nov. a methanol-utilizing bacterium isolated from the forest soil

  • Yan-Ru Cao
  • Qian Wang
  • Rong-Xian Jin
  • Shu-Kun Tang
  • Yi JiangEmail author
  • Wen-Xiang He
  • Hang-Xian Lai
  • Li-Hua Xu
  • Cheng-Lin Jiang
Article

Abstract

A Gram-negative, pink-pigmented, non-spore-forming rod shaped, methanol-utilizing bacterium, strain YIM 48816T, was isolated from forest soil collected from Sichuan province, China. Strain YIM 48816T can grow at 4–37°C, pH 5.0–7.0 and 0% NaCl (w/v). Based on 16S rRNA gene sequence similarity studies, it belonged to the genus Methylobacterium, and formed a phyletic line. The 16S rRNA gene sequence similarities were 96.2% to Methylobacterium mesophilicum DSM 1708T and 96.0% to Methylobacterium brachiatum DSM 19569T, and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 96.0%. The major menaquinones detected were Q-10 (97.14%) and Q-9 (2.86%). The major fatty acids were C18:1 ω7c (80.84%). The DNA G + C content was 66.2 mol%. It is apparent from the genotypic and phenotypic data that strain YIM 48816T belongs to a novel species of the genus Methylobacterium, for which the name Methylobacterium soli sp. nov. is proposed. The type strain is YIM 48816T (CCTCC AA 208027T = KCTC 22810T).

Keywords

Methylobacteriumsoli sp. nov Soil 16S rRNA gene 

Notes

Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 30900002 and No. 30560001), International Cooperative Key Project of Ministry of Science and Technology (2006DFA33550). Authors are grateful to Yong-xia Wang and Yun Wang for their help in the study.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Yan-Ru Cao
    • 1
    • 2
    • 3
  • Qian Wang
    • 2
    • 3
  • Rong-Xian Jin
    • 2
    • 3
  • Shu-Kun Tang
    • 2
    • 3
  • Yi Jiang
    • 2
    • 3
    Email author
  • Wen-Xiang He
    • 1
  • Hang-Xian Lai
    • 1
  • Li-Hua Xu
    • 2
    • 3
  • Cheng-Lin Jiang
    • 2
    • 3
  1. 1.College of Resources and EnvironmentNorthwest A&F UniversityXianyangChina
  2. 2.The Key Laboratory for Microbial Resources of the Ministry of EducationYunnan UniversityKunmingChina
  3. 3.Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of MicrobiologyYunnan UniversityKunmingChina

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