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Antonie van Leeuwenhoek

, Volume 99, Issue 3, pp 481–488 | Cite as

Bacillus hunanensis sp. nov., a slightly halophilic bacterium isolated from non-saline forest soil

  • Yi-Guang Chen
  • Di-Fei Hao
  • Qi-Hui Chen
  • Yu-Qin Zhang
  • Jian-Ben Liu
  • Jian-Wu He
  • Shu-Kun Tang
  • Wen-Jun Li
Original Paper

Abstract

A novel Gram-stain-positive, slightly halophilic, catalase- and oxidase-positive, endospore-forming, motile, aerobic, rod-shaped bacterium, designated strain JSM 081003T, was isolated from non-saline forest soil in Hunan Province, China. Growth occurred with 0.5–15% (w/v) NaCl (optimum 2–4%) at pH 6.5–10.5 (optimum pH 7.5–8.5) and at 5–40°C (optimum 30°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C15:0, anteiso-C15:0 and iso-C14:0. Strain JSM 081003T contained MK-7 as the predominant respiratory quinone, and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The genomic DNA G + C content of strain JSM 081003T was 40.9 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 081003T should be assigned to the genus Bacillus, and was related most closely to the type strains of Bacillus lehensis (sequence similarity 99.6%), Bacillus oshimensis (99.4%) and Bacillus patagoniensis (96.6%); lower than 96.0% sequence similarity was observed with other Bacillus species. The combination of phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the view that strain JSM 081003T represents a new species of the genus Bacillus, for which the name Bacillus hunanensis sp. nov. is proposed. The type strain is JSM 081003T (= DSM 23008T = KCTC 13711T).

Keywords

Bacillus hunanensis sp. nov. Halophilic Non-saline soil 

Notes

Acknowledgments

This work was supported by grants from the National Basic Research Program of China (2010CB833800), National Natural Science Foundation of China (NSFC) (30970007, 30970008), Key Laboratory of Ecotourism’s Application Technology of Hunan Province (SL2010Z01), Key Laboratory of Hunan Forest Products and Chemical Industry Engineering (JDZ200907) and State-level Public Welfare Scientific Research Institutes for Basic R&D Special Fund (IMBF200915). We are grateful to Mr Zhu-Xiang Liu and Mr Ke Huang for their excellent technical assistance.

Supplementary material

10482_2010_9512_MOESM1_ESM.doc (73 kb)
Supplementary material 1 (DOC 73 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Yi-Guang Chen
    • 1
    • 2
  • Di-Fei Hao
    • 5
  • Qi-Hui Chen
    • 1
    • 2
  • Yu-Qin Zhang
    • 4
  • Jian-Ben Liu
    • 1
    • 2
  • Jian-Wu He
    • 1
    • 2
  • Shu-Kun Tang
    • 3
  • Wen-Jun Li
    • 3
  1. 1.Key Laboratory of Ecotourism’s Application Technology of Hunan Province, College of Biology and Environmental SciencesJishou UniversityJishouPeople’s Republic of China
  2. 2.Key Laboratory of Ecotourism’s Application Technology of Hunan Province, College of Chemistry and Chemical EngineeringJishou UniversityJishouPeople’s Republic of China
  3. 3.The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of MicrobiologyYunnan UniversityKunmingPeople’s Republic of China
  4. 4.Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingPeople’s Republic of China
  5. 5.Department of Food EngineeringJiangsu Food Science CollegeHuaianPeople’s Republic of China

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