Comparison of methods for the extraction of DNA from stream epilithic biofilms
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This study compares how different DNA extraction methods influence the quantity and quality of DNA yields from stream epilithic biofilms. Interpretations of bacterial community structure, using ARISA, revealed increased variability among samples processed using commercially-available kits, which also yielded lower DNA concentrations than other methods tested.
KeywordsBacterial DNA extraction kit ARISA
This work was funded by the Foundation for Research, Science and Technology, New Zealand (Grant UOAX306). We thank Kristine Boxen for her kind assistance with the ARISA of bacterial DNA.
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