Antonie van Leeuwenhoek

, Volume 98, Issue 4, pp 567–571 | Cite as

Comparison of methods for the extraction of DNA from stream epilithic biofilms

  • Gavin Lear
  • Yimin Dong
  • Gillian Lewis
Short Communication


This study compares how different DNA extraction methods influence the quantity and quality of DNA yields from stream epilithic biofilms. Interpretations of bacterial community structure, using ARISA, revealed increased variability among samples processed using commercially-available kits, which also yielded lower DNA concentrations than other methods tested.


Bacterial DNA extraction kit ARISA 



This work was funded by the Foundation for Research, Science and Technology, New Zealand (Grant UOAX306). We thank Kristine Boxen for her kind assistance with the ARISA of bacterial DNA.

Supplementary material

10482_2010_9464_MOESM1_ESM.doc (74 kb)
Supplementary material 1 (DOC 73 kb)


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Soil and Physical SciencesLincoln UniversityLincolnNew Zealand
  2. 2.School of Biological SciencesThe University of AucklandAucklandNew Zealand

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