Rapid degradation of N-3-oxo-acylhomoserine lactones by a Bacillus cereus isolate from Malaysian rainforest soil
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A bacterial strain, KM1S, was isolated from a Malaysian rainforest soil sample by using a defined enrichment medium that specifically facilitates selection of quorum quenching bacteria. KM1S was clustered closely to Bacillus cereus by 16S ribosomal DNA sequence analysis. It degraded N-3-oxo-hexanoyl homoserine lactone and N-3-oxo-octanoyl homoserine lactone in vitro rapidly at 4.98 and 6.56 μg AHL h−1 per 109 CFU/ml, respectively, as determined by the Rapid Resolution Liquid Chromatography. The aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of N-acylhomoserine lactones, of KM1S was amplified and cloned. Sequence analysis indicated the presence of the motif 106HXDH-59 amino acids-H169-21 amino acids-D191 for N-acylhomoserine lactone lactonases.
KeywordsBacilluscereus KG medium Quorum sensing Quorum quenching N-acylhomoserine lactone Rainforest soil
We gratefully acknowledge the support of three grants from the University of Malaya, namely, Vot F Research Grant (FS260/2008C), Research University Grants (TA048-2008A, RG003/09BIO), both to KG Chan, and Postgraduate Research Grant (PS256/2008A) to CS Wong.
- Chan KG, Tiew SZ, Ng CC (2007) Rapid isolation method of soil bacilli and screening of their quorum quenching activity. Asia Pac J Mol Biol Biotechnol 15:153–156Google Scholar
- McClean KH, Winson MK, Fish L, Taylor A, Chhabra SR, Cámara M, Daykin M, Lamb JH, Swift S, Bycroft BW, Stewart GSAB, Williams P (1997) Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology 143:3703–3711CrossRefPubMedGoogle Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, USAGoogle Scholar
- Swift S, Winson MK, Chan PF, Bainton NJ, Birdsall M, Reeves PJ, Chhabra SR, Hill PJ, Throup JP, Bycroft BW, Salmond GPC, Williams P, Stewart GSAB (1993) A novel strategy for the isolation of LuxI homologs: evidence for the widespread distribution of a LuxR:LuxI superfamily in enteric bacteria. Mol Microbiol 10:511–520CrossRefPubMedGoogle Scholar
- Yates EA, Philipp B, Buckley C, Atkinson S, Chhabra SR, Sockett RE, Goldner M, Dessaux Y, Cámara M, Smith H, Williams P (2002) N-acylhomoserine Lactones undergo lactonolysis in a pH-, temperature-, and acyl chain length-dependent manner during growth of Yersinia pseudotuberculosis and Pseudomonas aeruginosa. Infect Immun 70:5635–5646CrossRefPubMedGoogle Scholar