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Antonie van Leeuwenhoek

, Volume 98, Issue 3, pp 299–305 | Cite as

Rapid degradation of N-3-oxo-acylhomoserine lactones by a Bacillus cereus isolate from Malaysian rainforest soil

  • Kok-Gan Chan
  • Cheng-Siang Wong
  • Wai-Fong Yin
  • Choon-Kook Sam
  • Chong-Lek Koh
Original Paper

Abstract

A bacterial strain, KM1S, was isolated from a Malaysian rainforest soil sample by using a defined enrichment medium that specifically facilitates selection of quorum quenching bacteria. KM1S was clustered closely to Bacillus cereus by 16S ribosomal DNA sequence analysis. It degraded N-3-oxo-hexanoyl homoserine lactone and N-3-oxo-octanoyl homoserine lactone in vitro rapidly at 4.98 and 6.56 μg AHL h−1 per 109 CFU/ml, respectively, as determined by the Rapid Resolution Liquid Chromatography. The aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of N-acylhomoserine lactones, of KM1S was amplified and cloned. Sequence analysis indicated the presence of the motif 106HXDH-59 amino acids-H169-21 amino acids-D191 for N-acylhomoserine lactone lactonases.

Keywords

Bacilluscereus KG medium Quorum sensing Quorum quenching N-acylhomoserine lactone Rainforest soil 

Notes

Acknowledgements

We gratefully acknowledge the support of three grants from the University of Malaya, namely, Vot F Research Grant (FS260/2008C), Research University Grants (TA048-2008A, RG003/09BIO), both to KG Chan, and Postgraduate Research Grant (PS256/2008A) to CS Wong.

Supplementary material

10482_2010_9438_MOESM1_ESM.pdf (11 kb)
Supplementary material 1 (PDF 10 kb)
10482_2010_9438_MOESM2_ESM.pdf (251 kb)
Supplementary material 2 (PDF 251 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Kok-Gan Chan
    • 1
    • 2
  • Cheng-Siang Wong
    • 1
    • 2
  • Wai-Fong Yin
    • 1
    • 2
  • Choon-Kook Sam
    • 3
  • Chong-Lek Koh
    • 3
  1. 1.Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia
  2. 2.Centre for Research in Biotechnology for Agriculture (CEBAR), Institute of Postgraduate StudiesUniversity of MalayaKuala LumpurMalaysia
  3. 3.Natural Sciences and Science Education AG, National Institute of EducationNanyang Technological UniversitySingaporeSingapore

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