Maintenance of previously uncultured freshwater archaea from anoxic waters under laboratory conditions
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Culture conditions for the maintenance of previously uncultured members of the Archaea thriving in anoxic water layers of stratified freshwater lakes are described. The proposed enrichment conditions, based on the use of defined medium composition and the maintenance of anoxia, have been proven effective for the maintenance of the archaeal community with virtually no changes over time for periods up to 6 months as revealed by a PCR-DGGE analysis. Phylotypes belonging to groups poorly represented in culture collections such as the Deep-Sea Hydrothermal Vent Euryarchaeota (DHVE) and the Miscellaneous Crenarchaeotic Group (MCG) were maintained and selectively enriched when compared to the correspondent indigenous planktonic archaeal community.
KeywordsArchaea Enrichment cultures Freshwater Crenarchaeota Oxic/anoxic interfaces Stratified lakes
This work was supported by coordinated projects REN2003-08333 and CRENYC CGL2006-12058 from the Spanish Ministerio de Educación y Ciencia (MEC) to CMB and EOC. We thank A. Camacho and A. Picazo from the Institut Cavanilles de Biodiversitat i Biologia Evolutiva of the University of Valencia for their assistance and valuable support during the sampling campaign in Cuenca. A. Plasencia and M. Llirós benefit from a FI doctoral fellow from the Generalitat de Catalunya and from a FPI doctoral fellow from the Spanish MEC, respectively.
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