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Antonie van Leeuwenhoek

, Volume 96, Issue 4, pp 537–544 | Cite as

Cell yields and fermentation responses of a Salmonella Typhimurium poultry isolate at different dilution rates in an anaerobic steady state continuous culture

  • Kingsley D. Dunkley
  • Todd R. Callaway
  • Corliss O’Bryan
  • Megan M. Kundinger
  • Claudia S. Dunkley
  • Robin C. Anderson
  • David J. Nisbet
  • P. G. Crandall
  • Steven C. Ricke
Original Paper

Abstract

The objectives of these studies were to determine cell yield and fermentation responses of a Salmonella enterica serovar Typhimurium poultry isolate using various dilution rates in steady state continuous culture incubations. S. enterica Typhimurium cells were propagated in continuous cultures with a total volume of 0.50 l of Luria Bertani medium containing 0.1% glucose. Dilution rates from 0.0125 to 1.44/h were used. Cell protein concentration generally increased linearly with increased dilution rate up to a rate of 0.54/h and declined at the higher dilution rates. Glucose consumption gave a similar pattern to cell protein concentration by declining at the three highest dilution rates. Short chain fatty acid production was inconsistently influenced by dilution rate. Acetate, the most predominant fatty acid produced, declined at the higher dilution rates, as did propionate. Ammonia production remained stable at the lowest dilution rates, but increased significantly at a dilution rates above 0.27/h.

Keywords

Salmonella Continuous culture Fermentation Glucose yield 

Notes

Acknowledgments

These studies were conducted with funds from USDA-NRI Grant # 2001-02675 administered by Texas Agricultural Experimental Station. Thanks to Albert Blanks for technical assistance, Cassie Woodward and Dr. W. K. Kim for data analysis and helpful discussion for preparation of the manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Kingsley D. Dunkley
    • 1
    • 2
  • Todd R. Callaway
    • 3
  • Corliss O’Bryan
    • 4
  • Megan M. Kundinger
    • 1
    • 5
  • Claudia S. Dunkley
    • 1
    • 6
  • Robin C. Anderson
    • 3
  • David J. Nisbet
    • 3
  • P. G. Crandall
    • 4
  • Steven C. Ricke
    • 1
    • 4
  1. 1.Department of Poultry ScienceTexas A&M UniversityCollege StationUSA
  2. 2.Abraham Baldwin Agricultural CollegeTiftonUSA
  3. 3.USDA-ARSSouthern Plains Agricultural Research Center, Food and Feed Safety Research UnitCollege StationUSA
  4. 4.Department of Food ScienceUniversity of ArkansasFayettevilleUSA
  5. 5.Midstate Technical CollegeMarshfieldUSA
  6. 6.Department of Poultry ScienceThe University of GeorgiaTiftonUSA

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