Antonie van Leeuwenhoek

, 96:441 | Cite as

Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

  • Chai-Hoon Khoo
  • Yoke-Kqueen Cheah
  • Learn-Han Lee
  • Jiun-Horng Sim
  • Noorzaleha Awang Salleh
  • Shiran Mohd Sidik
  • Son Radu
  • Sabrina Sukardi
Original Paper

Abstract

The increased occurrence of Salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. This study is aimed to detect the prevalence of twenty different virulence factors among Salmonella enterica strains isolated from poultry and local indigenous vegetables in Malaysia via an optimized, rapid and specific multiplex PCR assay. The assay encompasses a total of 19 Salmonella pathogenicity islands genes and a quorum sensing gene (sdiA) in three multiplex reaction sets. A total of 114 Salmonella enterica isolates belonging to 38 different serovars were tested. Each isolate in under this study was found to possess up to 70% of the virulence genes tested and exhibited variable pathogenicity gene patterns. Reproducibility of the multiplex PCR assay was found to be 100% and the detection limit of the optimized multiplex PCR was tested with lowest detectable concentration of DNA 0.8 pg μl−1. This study demonstrated various Salmonella pathogenicity island virulence gene patterns even within the same serovar. This sets of multiplex PCR system provide a fast and reliable typing approach based on Salmonella pathogenicity islands, thus enabling an effective monitoring of emerging pathogenic Salmonella strains as an additional tool in Salmonella surveillance studies.

Keywords

Virulotyping Salmonellaenterica Salmonella pathogenicity islands (SPIs) Multiplex PCR 

Notes

Acknowledgments

The authors thank the Department of Chemistry, Malaysia for collaboration and providing the Salmonella isolates in this study. This study was supported by Fundamental Research Grant Scheme (04-01-07-098) provided from the Ministry of Higher Education, Malaysia.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Chai-Hoon Khoo
    • 1
  • Yoke-Kqueen Cheah
    • 1
  • Learn-Han Lee
    • 1
  • Jiun-Horng Sim
    • 1
  • Noorzaleha Awang Salleh
    • 2
  • Shiran Mohd Sidik
    • 3
  • Son Radu
    • 4
  • Sabrina Sukardi
    • 1
  1. 1.Department of Biomedical Science, Faculty of Medicine and Health SciencesUniversity Putra MalaysiaSerdangMalaysia
  2. 2.Department of Chemistry MalaysiaSection of Biotechnology, Unit MicrobiologyPetaling JayaMalaysia
  3. 3.Department of Pathology, Faculty of Medicine and Health SciencesUniversity Putra MalaysiaSerdangMalaysia
  4. 4.Department of Food Science, Faculty of Food Science and TechnologyUniversity Putra MalaysiaSerdangMalaysia

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