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Antonie van Leeuwenhoek

, Volume 96, Issue 1, pp 63–70 | Cite as

Arthrobacter halodurans sp. nov., a new halotolerant bacterium isolated from sea water

  • Yi-Guang ChenEmail author
  • Shu-Kun Tang
  • Yu-Qin Zhang
  • Zhao-Yang Li
  • Lang-Bo Yi
  • Yong-Xia Wang
  • Wen-Jun Li
  • Xiao-Long Cui
Original Paper

Abstract

A novel Gram-positive, halotolerant, non-sporulating, non-motile, catalase-positive, oxidase-negative and aerobic bacterium, designated strain JSM 078085T, was isolated from sea water collected from the South China Sea. Strain JSM 078085T exhibited a rod-coccus growth cycle and produced a yellow pigment. The strain was able to grow in the presence of 0–12% (w/v) NaCl and at pH 6.0–9.5 and 4–35°C; optimum growth was observed at pH 7.0 and 25–30°C in the absence of NaCl. The peptidoglycan type was A4α (l-Lys–l-Ala–l-Glu). Cell-wall sugars contained galactose and glucose. Strain JSM 078085T contained menaquinone MK-9(H2) as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major polar lipids. The major cellular fatty acids were anteiso-C15:0, iso-C15:0 and anteiso-C17:0 and the DNA G + C content was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 078085T should be assigned to the genus Arthrobacter, being most closely related to the type strain of Arthrobacter rhombi (sequence similarity 97.1%), and the two strains formed a distinct lineage in the phylogenetic tree. The level of DNA–DNA relatedness between strain JSM 078085T and the type strain of Arthrobacter rhombi was 10.6%. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 078085T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter halodurans sp. nov. is proposed. The type strain is JSM 078085T (=DSM 21081T=KCTC 19430T).

Keywords

Arthrobacter halodurans sp. nov Halotolerant South China Sea Naozhou Island 

Notes

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NSFC) (30860013, 30860002, 30660004), Jishou University (jsdxkyzz200811), the Ministry of Science and Technology, PR China (2006BAE01A01-9, 863 Program, no. 2007AA021306), Key Projects of International Cooperation (2007DFB31620), the Yunnan Provincial Sciences and Technology Department (2005PY01-1, 2006C0006 M) and Yunnan University (2008YB005). We are grateful to Mr. Huai-Dong Xiao and Mr. Ke Huang for their excellent technical assistance.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Yi-Guang Chen
    • 1
    • 2
    • 3
    Email author
  • Shu-Kun Tang
    • 2
    • 3
  • Yu-Qin Zhang
    • 2
    • 3
    • 4
  • Zhao-Yang Li
    • 1
  • Lang-Bo Yi
    • 1
  • Yong-Xia Wang
    • 2
    • 3
  • Wen-Jun Li
    • 2
    • 3
    • 5
  • Xiao-Long Cui
    • 2
    • 3
  1. 1.Key Laboratory for Conservation and Utilization of Plant Resources of Hunan Province, College of Biology and Environmental SciencesJishou UniversityJishouPeople’s Republic of China
  2. 2.The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of MicrobiologyYunnan UniversityKunmingPeople’s Republic of China
  3. 3.Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of MicrobiologyYunnan UniversityKunmingPeople’s Republic of China
  4. 4.Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingPeople’s Republic of China
  5. 5.Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouPeople’s Republic of China

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