Kazachstania wufongensis sp. nov., an ascosporogenous yeast isolated from soil in Taiwan

  • Ching-Fu Lee
  • Cheng-Hsu Yao
  • Yi-Ru Liu
  • Shuh-Sen Young
  • Ku-Shang Chang
Original Paper

Abstract

A new yeast species, Kazachstania wufongensis, is proposed in this paper based on six strains isolated from soil in Taiwan. The species may produce one to four ellipsoidal ascospores in each ascus, directly transformed from diploid cells. Genus assignment and distinction of the species from other recognized species of Kazachstania is based on morphological and physiological characteristics, and on phylogenetic analysis of nucleotide sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene. Sequence analysis of the D1/D2 domains of the LSU rRNA gene reveals that K. wufongensis is a member of the Kazachstania exigua complex, and its phylogenetically closest relatives are K. exigua, K. barnettii, K. bulderi, and K. turicensis. The species can be further differentiated from the other phylogenetically related species based on internal transcribed spacer sequence and electrophoretic karyotype. Therefore, the new species Kazachstania wufongensis sp. nov. is proposed. The type strain of this new species, which was isolated from forest soil in Wufong, Hsinchu, Taiwan, is FN21S03T (=CBS 10886T = BCRC 23138T).

Keywords

Yeast New species Systematics Kazachstania wufongensis 

Notes

Acknowledgments

This work was supported by a Grant-in-Aid (NSC 95-2621-B-134-001) from the National Science Council, Executive Yuan, Taiwan.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Ching-Fu Lee
    • 1
  • Cheng-Hsu Yao
    • 1
  • Yi-Ru Liu
    • 1
  • Shuh-Sen Young
    • 1
  • Ku-Shang Chang
    • 2
  1. 1.Department of Applied ScienceNational Hsinchu University of EducationHsinchuTaiwan
  2. 2.Department of Food ScienceYuanpei UniversityHsinchuTaiwan

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