Kazachstania wufongensis sp. nov., an ascosporogenous yeast isolated from soil in Taiwan
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A new yeast species, Kazachstania wufongensis, is proposed in this paper based on six strains isolated from soil in Taiwan. The species may produce one to four ellipsoidal ascospores in each ascus, directly transformed from diploid cells. Genus assignment and distinction of the species from other recognized species of Kazachstania is based on morphological and physiological characteristics, and on phylogenetic analysis of nucleotide sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene. Sequence analysis of the D1/D2 domains of the LSU rRNA gene reveals that K. wufongensis is a member of the Kazachstania exigua complex, and its phylogenetically closest relatives are K. exigua, K. barnettii, K. bulderi, and K. turicensis. The species can be further differentiated from the other phylogenetically related species based on internal transcribed spacer sequence and electrophoretic karyotype. Therefore, the new species Kazachstania wufongensis sp. nov. is proposed. The type strain of this new species, which was isolated from forest soil in Wufong, Hsinchu, Taiwan, is FN21S03T (=CBS 10886T = BCRC 23138T).
KeywordsYeast New species Systematics Kazachstania wufongensis
This work was supported by a Grant-in-Aid (NSC 95-2621-B-134-001) from the National Science Council, Executive Yuan, Taiwan.
- Kurtzman CP, Robnett CJ, Wart JM, Brayton C, Gorelick P, Walsh TJ (2005) Multigene phylogenetic analysis of pathogenic Candida species in the Kazachstania (Arxiozyma) telluris complex and description of their ascosporic states as Kazachstania bovina sp. nov., K. heterogenica sp. nov., K. pintolopesii sp. nov., and K. slooffiae sp. nov. J Clin Microbiol 43:101–111PubMedCrossRefGoogle Scholar
- Lachance MA (1998) Kluyveromyces van derWalt emend. van der Walt. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 227–247Google Scholar
- Lee CF, Yao CH, Liu YR, Hsieh CW, Young SS (2009) Lachancea dasiensis sp. nov., an ascosporogenous yeast isolated from soil and leaves in Taiwan. Int J Syst Evol Microbiol (in press)Google Scholar
- Mikata K, Ueda-Nishimura K, Hisatomi T (2001) Three new species of Saccharomyces sensu lato van der Walt from Yaku Island in Japan: Saccharomyces naganishii sp. nov., Saccharomyces humaticus sp. nov. and Saccharomyces yakushimaensis sp. nov. Int J Syst Evol Microbiol 51:2189–2198PubMedGoogle Scholar
- Vaughan-Martini A, Martini A (1998) Saccharomyces Meyen ex Reess. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 358–371Google Scholar
- White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide for methods and applications. Academic Press, New York, pp 315–322Google Scholar
- Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 77–102Google Scholar