ArsR arsenic-resistance regulatory protein from Cupriavidus metallidurans CH34
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The Cupriavidus metallidurans CH34 arsR gene, which is part of the arsRIC 2 BC 1 HP operon, and its putative arsenic-resistance regulatory protein were identified and characterized. The arsenic-induced transcriptome of C. metallidurans CH34 showed that the genes most upregulated in the presence of arsenate were all located within the ars operon, with none of the other numerous heavy metal resistance systems present in CH34 being induced. A transcriptional fusion between the luxCDABE operon and the arsR promoter/operator (P/O) region was used to confirm the in vivo induction of the ars operon by arsenite and arsenate. The arsR gene was cloned into expression vectors allowing for the overexpression of the ArsR protein as either his-tagged or untagged protein. The ability of the purified ArsR proteins to bind to the ars P/O region was analyzed in vitro by gel mobility shift assays. ArsR showed an affinity almost exclusively to its own ars P/O region. Dissociation of ArsR and its P/O region was metal dependent, and based on decreasing degrees of dissociation three groups of heavy metals could be distinguished: As(III), Bi(III), Co(II), Cu(II), Ni(II); Cd(II); Pb(II) and Zn(II), while no dissociation was observed in the presence of As(V).
KeywordsCupriavidus metallidurans CH34 Arsenic resistance ArsR Gene expression Metal binding
This work was supported by the US DOE Office of Science and Office of Basic Energy Sciences under Contract No. DE-AC-02-98CH10886. S. M. was supported by the AWM Ph.D grant from SCK.CEN.
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