Antonie van Leeuwenhoek

, 95:207 | Cite as

Alternative respiration of fungus Phycomyces blakesleeanus

  • M. Živić
  • J. Zakrzewska
  • M. Stanić
  • T. Cvetić
  • B. Živanović
Original Paper


Respiratory characteristics of germinating spores, developing mycelium and mitochondria of the fungus Phycomyces blakesleeanus were investigated by means of oxygen Clark-type electrode. The effects of respiratory inhibitors and metabolic compounds on oxygen consumption were tested. It was demonstrated that P. blakesleeanus apart of cyanide-sensitive respiration, CSR, possess alternative respiration, (cyanide-resistant respiration, CRR) which is constitutive and whose capacity decreases during development. Maximum is observed for activated spores where CRR capacity is significantly greater than CSR. After treatment with antimycin A, a third type of respiration insensitive to antimycin A and low concentration of SHAM (sufficient for inhibition of CRR), but sensitive to cyanide and high concentration of SHAM, has been expressed.


Alternative oxidase Cyanide resistant respiration Development Phycomyces blakesleeanus 



The authors are grateful to Prof. Paul Galland for providing Phycomyces strains and critical reading of this manuscript. This work was supported by Serbian Ministry of Science grant No. 143016B.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • M. Živić
    • 1
  • J. Zakrzewska
    • 2
  • M. Stanić
    • 1
  • T. Cvetić
    • 3
  • B. Živanović
    • 4
  1. 1.Department of BiologyState University of Novi PazarNovi PazarSerbia
  2. 2.Institute of General and Physical ChemistryBelgradeSerbia
  3. 3.Institute of Botany, Faculty of BiologyUniversity of BelgradeBelgradeSerbia
  4. 4.Institute for Multidisciplinary ResearchBelgradeSerbia

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