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Antonie van Leeuwenhoek

, Volume 94, Issue 4, pp 573–579 | Cite as

Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans

  • Pavel ŠvecEmail author
  • Dana Nováková
  • Lenka Žáčková
  • Martina Kukletová
  • Ivo Sedláček
Original Paper

Abstract

Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)5 primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)5-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter® microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)5 primer to be a fast and reliable method for identification of S. mutans.

Keywords

Dental caries (GTG)5-PCR Identification Automated ribotyping Streptococcus mutans 

Notes

Acknowledgments

Financial support from the Ministry of Education, Youth, and Sports of the Czech Republic projects 1M0528 and MSM0021622416 is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Pavel Švec
    • 1
    Email author
  • Dana Nováková
    • 1
  • Lenka Žáčková
    • 2
  • Martina Kukletová
    • 3
  • Ivo Sedláček
    • 1
  1. 1.Czech Collection of Microorganisms, Institute of Experimental BiologyMasaryk UniversityBrnoCzech Republic
  2. 2.Department of PedodonticsChildren’s Teaching HospitalBrnoCzech Republic
  3. 3.Centre for Dental and Craniofacial ResearchMasaryk UniversityBrnoCzech Republic

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