Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans
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Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)5 primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)5-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter® microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)5 primer to be a fast and reliable method for identification of S. mutans.
KeywordsDental caries (GTG)5-PCR Identification Automated ribotyping Streptococcus mutans
Financial support from the Ministry of Education, Youth, and Sports of the Czech Republic projects 1M0528 and MSM0021622416 is gratefully acknowledged.
- Hardie JM, Whiley RA (1995) The genus Streptococcus. In: Wood BJB, Holzapfel WH (eds) The genera of lactic acid bacteria: the lactic acid bacteria, vol 2. Chapman & Hall, Glasgow, pp 55–124Google Scholar
- Marsh P, Martin MV (eds) (1999) The resident oral microflora. In: Oral Microbiology, 4th edn. Wright, Oxford, pp 17–33Google Scholar
- Pot B, Vandamme P, Kersters K (1994) Analysis of electrophoretic whole-organism protein fingerprints. In: Goodfellow M, O’Donnell AG (eds) Chemical methods in prokaryotic systematics. Wiley, Chichester, pp 493–521Google Scholar