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Antimicrobial susceptibility and invasive ability of Staphylococcus aureus isolates from mastitis from dairy backyard systems

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Abstract

Fifteen (15) backyard farms were investigated to determine the antimicrobial susceptibility and invasion ability of S. aureus isolates from cows with subclinical mastitis in México. A total of 106 cows were sampled and 31 S. aureus isolates were recovered. S. aureus isolates were resistant to penicillin class antibiotics and susceptible to gentamicin and cetyltrimethylammonium bromide. STA9 and STA13 isolates were resistant to erythromycin (MIC > 25 μg/ml) and lincomycin (STA13, MIC > 25 μg/ml; STA9, MIC > 100 μg/ml). STA9 isolate harbors the erm(B) and msr(A) genes, whereas STA13 isolate harbors the erm(C) gene. STA9 and STA13 isolates contains the lnu(A) gene. Only 5 isolates (STA11, STA13, STA14, STA15 and STA21) were able to internalize in bovine mammary epithelial cells. These results indicate that S. aureus isolates from dairy backyard farms showed differences in the antimicrobial susceptibility patterns and invasion ability in bovine mammary epithelial cells. This kind of evaluations should be performed in different dairy regions, since resistance patterns and isolate diversity vary on a per-region basis.

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Abbreviations

bMEC:

Bovine mammary epithelial cells

MLS:

Macrolides, lincosamide, and streptogramin antibiotics

QAC:

Quaternary ammonium compounds

CTAB:

Cetyltrimethylammonium bromide

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Acknowledgments

This work was supported by grants from COECyT-UMSNH (CB0702112_9 and CB0702138_1) and CONACyT (46400) to A.O.Z. and J.E.L.M. The authors thank to Dr. Alfonso Cárabez-Trejo (Neurobiology Institute, UNAM, México) for the electron microscopy micrographs and to Francisco Jesús Hidalgo Cervantes for the milk collection.

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Authors and Affiliations

  1. Centro Multidisciplinario de Estudios en Biotecnología-Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Apdo. Postal 53, Administración Chapultepec, C.P. 58262, Morelia, Michoacán, México

    Alejandra Ochoa-Zarzosa, Pedro D. Loeza-Lara, Francisco Torres-Rodríguez, Heber Loeza-Ángeles, Nidia Mascot-Chiquito, Sonia Sánchez-Baca & Joel E. López-Meza

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  1. Alejandra Ochoa-Zarzosa
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  2. Pedro D. Loeza-Lara
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  3. Francisco Torres-Rodríguez
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  4. Heber Loeza-Ángeles
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  5. Nidia Mascot-Chiquito
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  6. Sonia Sánchez-Baca
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  7. Joel E. López-Meza
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Correspondence to Joel E. López-Meza.

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Ochoa-Zarzosa, A., Loeza-Lara, P.D., Torres-Rodríguez, F. et al. Antimicrobial susceptibility and invasive ability of Staphylococcus aureus isolates from mastitis from dairy backyard systems. Antonie van Leeuwenhoek 94, 199–206 (2008). https://doi.org/10.1007/s10482-008-9230-6

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  • DOI: https://doi.org/10.1007/s10482-008-9230-6

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