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Antonie van Leeuwenhoek

, Volume 93, Issue 3, pp 241–248 | Cite as

A comparative study on the phylogenetic diversity of culturable actinobacteria isolated from five marine sponge species

  • Haitao Zhang
  • Wei Zhang
  • Yan Jin
  • Meifang Jin
  • Xingju Yu
Original Paper

Abstract

A cultivation-based approach was employed to compare the culturable actinobacterial diversity associated with five marine sponge species (Craniella australiensis, Halichondria rugosa, Reniochalina sp., Sponge sp., and Stelletta tenuis). The phylogenetic affiliation of the actinobacterial isolates was assessed by 16S rDNA-RFLP analysis. A total of 181 actinobacterial strains were isolated using five different culture media (denoted as M1–M5). The type of medium exhibited significant effects on the number of actinobacteria recovered, with the highest number of isolates on M3 (63 isolates) and the lowest on M1 (12 isolates). The genera isolated were also different, with the recovery of three genera on M2 and M3, and only a single genus on M1. The number of actinobacteria isolated from the five sponge species was significantly different, with a count of 83, 36, 30, 17, and 15 isolates from S. tenuis, H. rugosa, Sponge sp., Reniochalina sp., and C. australiensis, respectively. M3 was the best isolation medium for recovery of actinobacteria from S. tenuis, H. rugosa, and Sponge sp., while no specific medium preference was observed for the recovery of actinobacteria from Reniochalina sp., and C. australiensis. The RFLP fingerprinting of 16S rDNA genes digested with HhaI revealed six different patterns, in which 16 representative 16S rDNAs were fully sequenced. Phylogenetic analysis indicated that 12 strains belong to the group Streptomyces, three strains belong to Pseudonocardia, and one strain belongs to Nocardia. Two strains C14 (from C. australiensis) and N13 (from Sponge sp.) have only 96.26% and 96.27% similarity to earlier published sequences, and are therefore potential candidates for new species. The highest diversity of three actinobacteria genera was obtained from Sponge sp., though the number of isolates was low. Two genera of actinobacteria, Streptomyces, and Pseudonocardia, were isolated from both S. tenuis and C. australiensis. Only the genus of Streptomyces was isolated from H. rugosa and Reniochalina sp. Sponge species have been demonstrated here to vary as sources of culturable actinobacterial diversity, and the methods for sampling such diversity presented may be useful for improved sampling of such diversity.

Keywords

Actinobacteria Nocardia Pseudonocardia RFLP Sponge Streptomyces 16S rDNA sequencing 

Notes

Acknowledgements

The authors wish to acknowledge financial support from “Innovation Fund” of Dalian Institute of Chemical Physics, “973 Key Basic Science Research Program of China” (2003CB716001), and “863 Hi-Tech Research and Development Program of China” (2006AA09Z435). Dr. K. Manmadhan’s helps in English revision is greatly appreciated. We appreciate Professor Jinhe Li, at Qingdao Institute of Oceanology, Chinese Academy of Sciences for the sponge identification.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Haitao Zhang
    • 1
    • 2
  • Wei Zhang
    • 1
    • 3
  • Yan Jin
    • 1
  • Meifang Jin
    • 1
  • Xingju Yu
    • 1
  1. 1.Marine Bioproducts Engineering GroupDalian Institute of Chemical Physics, Chinese Academy of SciencesDalianChina
  2. 2.Graduate School of the Chinese Academy of SciencesChinese Academy of SciencesBeijingChina
  3. 3.Department of Medical Biotechnology, School of MedicineFlinders UniversityAdelaideAustralia

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