Antonie van Leeuwenhoek

, Volume 92, Issue 1, pp 95–100 | Cite as

Synergistic bactericidal activity of Eremanthus erythropappus oil or β-bisabolene with ampicillin against Staphylococcus aureus

  • Andréa M. A. NascimentoEmail author
  • Maria G. L. Brandão
  • Gabriel B. Oliveira
  • Isabel C. P. Fortes
  • Edmar Chartone-Souza
Original Paper


The activity of Eremanthus erythropappus oil (EO) and some of its compounds and their potential synergistic interaction with ampicillin against different strains of Staphylococcus aureus were investigated. Determination of chemical composition of EO by gas chromatography-mass spectrometry (GC/MS) and bioguided chemical fractionation led to the identification of β-bisabolene as the main active compound. A synergistic bactericidal activity of EO or β-bisabolene with ampicillin against Staphylococcus aureus was observed in a time-kill assay. EO and β- bisabolene have the potential to restore the effectiveness of ampicillin against resistant S. aureus.


Eremanthuserythropappus Eremanthus erythropappus oil β-bisabolene Staphylococcus aureus Ampicillin Synergism 



Eremanthus erythropappus oil


Mueller-Hinton broth



The authors are indebted to Givaudan Roure do Basil (São Paulo, Brazil) for supplying the authentic volatile compounds. This work was supported by a FAPEMIG grant CBB-1106/03.


  1. Adams RP (2001) Identification of essential oil components by gas chromatography mass spectrometry. Allured Pub. Corp., Carol Stream, IllinoisGoogle Scholar
  2. Ammon HPT, Sabieraj J, Kamille KR (1996) Mechanismus derantiphlogistischen wirkung von Kamillenextract und Inhaltsstoffen. Dtsch Apoth Ztg 36:1821–1834Google Scholar
  3. Braga LC, Leite AAM, Xavier KGS, Takahashi JA, Bemquerer MP, Chartone-Souza E, Nascimento AMA (2005) Synergic interaction between pomegranate extract and antibiotics against Staphylococcus aureus. Can J Microbiol 51:541–547PubMedCrossRefGoogle Scholar
  4. Braun NA, Meier M, Kohlenberg B, Hammeschmidt FJ (2003) Two new bisabolene diols from the stem wood essential oil of Vanillosmopsis erytropappa Schultz-Bi (Asteraceae). J Essent Oil Res 15:139–142Google Scholar
  5. Canalle R, Burim RV, Callegari JLL, Takahashi CS (2001) Assessment of the cytotoxic and clastogenic activities of the sesquiterpene lactone lynchnopholide in mammalian cells in vitro and in vivo. Cancer Detect Prev 25:93–101PubMedGoogle Scholar
  6. Darwish RM, Aburjai T, Al-Khalil S, Mahafzah A (2002) Screening of antibiotic resistant inhibitors from local plant materials against two different strains of Staphylococcus aureus. J Ethnopharmacol 79:359–364PubMedCrossRefGoogle Scholar
  7. Gouvinden-Soulange J, Magan N, Gurib-Fakin A, Gauvin A, Smadja J, Kodja H (2004) Chemical composition and in vitro antimicrobial activities of the essential oils from endemic Psidia species growing in Mauritius. Biol Pharm Bull 27:1814–1818CrossRefGoogle Scholar
  8. Jakolev O, Isaac I, Thiemer K, Kunde R (1979) Pharmacological investigations with compounds of chamomile – new investigations on the antiphlogistic effects of α- bisabolol and bisabolol oxides. Planta Med 35:125–140CrossRefGoogle Scholar
  9. Kuroda M, Ohta T, Uchiyama I, Baba T, Yuzawa H, Kobayashi I, Cui L, Oguchi A, Aoki K-I, Nagai Y, Lian J, Ito T, Kanamori M, Matsumaru H, Maruyama A, Murakami H, Hosoyama A, Mizutani-Ui Y, Takahashi NK, Sawano T, Inoue R-I, Kaito C, Sekimizu K, Hirakawa H, Kuhara S, Goto S, Yabuzaki J, Kanehisa M, Yamashita A, Oshima K, Furuya K, Yoshino C, Shiba T, Hattori M (2002) Whole genome sequencing of methicillin-resistant Staphylococcus aureus. Lancet 357:1225–1240CrossRefGoogle Scholar
  10. Lima PDDB, Garcia M, Rabi JA (1985) Selective extraction of α-methylene-γ-lactones Reinvestigation of Vanillosmopsis erytropappus. J Nat Prod 48:986–988CrossRefGoogle Scholar
  11. Lopes JNC, Lopes JLC, Vichnewsky WR, Dioneia C, Gottlieb OR (1991) Chemical variability of Vanillosmopsis erytropappa. Anais da Academia Brasileira de Ciências 63:21–22Google Scholar
  12. Mackay ML, Milne K, Gould IM (2000) Comparison of methods for assessing synergic antibiotic interactions. Int J Antimicrob Agents 15:125–129PubMedCrossRefGoogle Scholar
  13. Mendes MN, Queiroz RO, Grandi TSM, Anjos AMG, Oliveira AB, Zani CL (1999) Screening of Asteraceae (Compositae) plant extracts for molluscicidal activity. Mem I Oswaldo Cruz 94:411–412Google Scholar
  14. Menezes AMS, Almeida FRC, Rao VSN (1990). Anti-inflammatory activity of the essential oil of Vanillosmopsis arborea. Fitoterapia 61:252–254Google Scholar
  15. Nascimento GGF, Locatelli J, Freitas PC, Silva GL (2000) Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistance bacteria. Braz J Microbiol 31:247–256Google Scholar
  16. O’Connell NH, Humphreys H., Pidgeon C, Smyth EG (2003) Absence of risk factors for community MRSA. Clin Microbiol Infec 9:578–579CrossRefGoogle Scholar
  17. Simic N, Palic R, Randjelovc V (2005) Composition and antibacterial activity of Achillea clypeolata essential oil. Flavour Frag J 20:127–130CrossRefGoogle Scholar
  18. Smith-Palmer A, Stewart J, Fyfe L (1998) Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. Lett Food Microbiol 26:118–122CrossRefGoogle Scholar
  19. Swigar AA, Silverstein RM (1981) Monoterpenes. Aldrich Chemical Co. Inc., Milwaukee, WIGoogle Scholar
  20. Vichnewski W, Takahashi AM, Nasi AMT, Rodrigues DC, Goncalves G, Dias DA, Lopes JNC, Goedken VL, Gutiérrez AB Herz W (1989) Sesquiterpene lactones and other constituents from Eremanthus seidelii, E. goyazensis and Vanillosmopsis erythropappa. Phytochemistry 28:1441–1451CrossRefGoogle Scholar
  21. Von Sydow E. (1963) Mass spectrometry of terpenes monoterpenes alcohol. Acta Chem Scand 17:2504–2512Google Scholar
  22. Von Sydow E. (1964) Mass spectrometry of terpenes monoterpenes aldehydes and ketones. Acta Chem Scand 18:1099–1101CrossRefGoogle Scholar
  23. Yam TS, Hamilton-Müeller JMT, Shah S (1998) The effect of a component of tea (Camellia sinensis) on methicillin resistance, PBP2’ Synthesis, and β-lactamase production in Staphylococcus aureus. J Antimicrob Chemoth 42:211–216CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Andréa M. A. Nascimento
    • 1
    Email author
  • Maria G. L. Brandão
    • 2
  • Gabriel B. Oliveira
    • 1
  • Isabel C. P. Fortes
    • 3
  • Edmar Chartone-Souza
    • 1
  1. 1.Departamento de Biologia GeralInstituto de Ciências Biológicas – Universidade Federal de Minas GeraisBelo HorizonteBrazil
  2. 2.Departamento de Produtos Farmacêuticos, Faculdade de FarmáciaUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  3. 3.Departamento de QuímicaInstituto de Ciências Exatas. Universidade Federal de Minas GeraisBelo HorizonteBrazil

Personalised recommendations