31P NMR study of polyphosphate levels during different growth phases of Phycomyces blakesleeanus
- 175 Downloads
The changes in relative polyphosphate content, estimated as the intensity ratio of core polyphosphate signal and intracellular inorganic phosphate signal from 31P NMR spectra, during the growth of Phycomyces blakesleeanus are reported. The ratio increases from 16 h to 28 h of growth, the minimum occurs at 32 h, followed by sharp increase up to 36 h, and a steady decrease afterwards. The changes in the biomass during mycelium growth showed steady increases, with a stagnation period between 32 h and 36 h during which a pronounced increase in the intensity ratio of core polyphosphates to intracellular inorganic phosphate signal occurred. The reduction of growth temperature from 22°C to 18°C significantly decreased the rate and intensity of growth, but the pattern of polyphosphate changes remained unchanged. The changes of the intensity ratio of core polyphosphates to intracellular inorganic phosphate signal are linked to characteristic stages of sporangiophore development. Analysis of core polyphosphates, intracellular inorganic phosphate and β-ATP signal intensities suggest the role of polyphosphates as an energy and/or a phosphate reserves during Phycomyces development.
KeywordsGrowth 31P NMR Phycomyces blakesleeanus Polyphosphate
Intracellular inorganic phosphate
Central (core) residues of PolyP
Terminal phosphates of PolyP
This work was supported by a grant from the Ministry of Science and Environmental Protection of the Republic of Serbia (projects No. 1934). We would like to thank Prof. Dr Željko Vučinić for useful suggestions during the preparation of this manuscript.
- Loughman BC, Ratcliff RG (1984) Nuclear magnetic resonance and the study of plants. In: Tinker PB, Lauchli A (eds) Advances in plant nutrition, vol 1. Praeger Publishers, New York NY, USA, pp 241–283Google Scholar
- Pfeffer PE, Shachar-Hill Y (1996) Plant/Microbe symbioses. In: Shachar-Hill Y, Pfeffer PE (eds) Nuclear magnetic resonance in plant biology. American Society of Plant Physiologist, Rockville, Maryland USA, pp 77–107Google Scholar
- Roberts JKM (1986) NMR methods for determination of intracellular pH. In: Linskens HF, Jackson JF (eds) Modern methods of plant analysis, Nuclear Magnetic Resonance, vol 2. Springer-Verlag, Berlin, Germany, pp 106–124Google Scholar
- Shabalin YA, Vagabov VM, Tsiomenko AB, Zemlianuhina OA, Kulaev IS (1977) Study of polyphosphate kinase activity in the yeast vacuoles. Biokhimiya (Moskow) 42:1642–1648Google Scholar
- Vagabov VM, Trilisenko LV, Shchipanova IN, Sibeldina LA, Kulaev IS (1998) Changes in inorganic polyphosphate length during growth of Saccharomyces cerevisiae. Microbiology (Moskow) 67:154–157Google Scholar
- Vagabov VM, Shabalin YA (1985) The detection in yeast a new fermentative system coupling synthesis of glycoproteins and inorganic condensed phosphates. In: Skryabin GK (eds) Problems of the biochemistry and physiology of microorganism. USSR Academy of Sciences, Pushchino, USSR, pp 167–175Google Scholar