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Antonie van Leeuwenhoek

, Volume 91, Issue 2, pp 169–177 | Cite as

31P NMR study of polyphosphate levels during different growth phases of Phycomyces blakesleeanus

  • Miroslav Živić
  • Joanna Zakrzewska
  • Milan Žižić
  • Goran Bačić
Original Paper

Abstract

The changes in relative polyphosphate content, estimated as the intensity ratio of core polyphosphate signal and intracellular inorganic phosphate signal from 31P NMR spectra, during the growth of Phycomyces blakesleeanus are reported. The ratio increases from 16 h to 28 h of growth, the minimum occurs at 32 h, followed by sharp increase up to 36 h, and a steady decrease afterwards. The changes in the biomass during mycelium growth showed steady increases, with a stagnation period between 32 h and 36 h during which a pronounced increase in the intensity ratio of core polyphosphates to intracellular inorganic phosphate signal occurred. The reduction of growth temperature from 22°C to 18°C significantly decreased the rate and intensity of growth, but the pattern of polyphosphate changes remained unchanged. The changes of the intensity ratio of core polyphosphates to intracellular inorganic phosphate signal are linked to characteristic stages of sporangiophore development. Analysis of core polyphosphates, intracellular inorganic phosphate and β-ATP signal intensities suggest the role of polyphosphates as an energy and/or a phosphate reserves during Phycomyces development.

Keywords

Growth 31P NMR Phycomyces blakesleeanus Polyphosphate 

Abbreviations

Pi

Intracellular inorganic phosphate

PolyP

Polyphosphate;

PPi

Central (core) residues of PolyP

PPt

Terminal phosphates of PolyP

UDPG

Uridine diphosphoglucose

Notes

Acknowledgements

This work was supported by a grant from the Ministry of Science and Environmental Protection of the Republic of Serbia (projects No. 1934). We would like to thank Prof. Dr Željko Vučinić for useful suggestions during the preparation of this manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Miroslav Živić
    • 1
  • Joanna Zakrzewska
    • 1
  • Milan Žižić
    • 2
  • Goran Bačić
    • 3
  1. 1.Institute of General and Physical ChemistryBelgradeSerbia and Montenegro
  2. 2.Center for Multidisciplinary Studies of Belgrade UniversityBelgradeSerbia and Montenegro
  3. 3.Faculty of Physical ChemistryUniversity of BelgradeBelgradeSerbia and Montenegro

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