Antonie van Leeuwenhoek

, Volume 90, Issue 3, pp 281–290 | Cite as

Sequential and structural analysis of [NiFe]-hydrogenase-maturation proteins from Desulfovibrio vulgaris Miyazaki F

  • Aruna Goenka Agrawal
  • Gerrit Voordouw
  • Wolfgang GärtnerEmail author


The complete primary structure of the hyn-region in the genome of Desulfovibrio vulgaris Miyazaki F (DvMF), encoding the [NiFe]-hydrogenase and two maturation proteins has been identified. Besides the formerly reported genes for the large and small subunits, this region comprises genes encoding an endopeptidase (HynC) and a putative chaperone (HynD). The complete genomic region covers 4086 nucleotides including the previously published upstream located promoter region and the sequences of the structural genes. A phylogenetic tree for both maturation proteins shows strongest sequential relationship to the orthologous proteins of Desulfovibrio vulgaris Hildenborough (DvH). Secondary structure prediction for HynC (168 aa, corresponding to a molecular weight of 17.9 kDa) revealed a practically identical arrangement of α-helical and β-strand elements between the orthologous protein HybD from E. coli and allowed a three-dimensional modelling of HynC on the basis of the formerly published structure of HybD. The putative chaperone HynD consists of 83 aa (molecular weight of 9 kDa) and shows 76% homology to DvH HynD. Preliminary experiments demonstrate that the operon is expressed under the control of its own promoter in Escherichia coli, although no further processing could be observed, providing evidence that additional proteins have to be involved in the maturation process. Accession numbers: DQ072852, HynC protein ID AAY90127, HynD protein ID AAY90128.


Endopeptidase [NiFe]-hydrogenase Operon structure Protein maturation process Three dimensional structure 



Desulfovibrio vulgaris Hildenborough


Desulfovibrio vulgaris Miyazaki F


large subunit


small subunit


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The support of Ulrich Krauss, U. Düsseldorf/FZ Jülich, in the structure-modelling of HynC is greatly acknowledged. We wish to thank also Dr. Reiner Hedderich from the Max-Planck-Institute for Terrestrial Microbiology, Marburg, for valuable suggestions during the experimental work. This work was supported by the Max-Planck-Society.


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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Aruna Goenka Agrawal
    • 1
  • Gerrit Voordouw
    • 2
  • Wolfgang Gärtner
    • 1
    Email author
  1. 1.Max-Planck-Institut für Bioanorganische ChemieMülheimGermany
  2. 2.Department of Biological SciencesThe University of CalgaryCalgaryCanada

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