Antonie van Leeuwenhoek

, Volume 89, Issue 3–4, pp 485–494

Genome-wide expression profile of the mnn2Δ mutant of Saccharomyces cerevisiae

  • Isaac Corbacho
  • Isabel Olivero
  • Stefan Hohmann
  • Per Sunnerhagen
  • Luis  M. Hernández


The MNN2 gene of S. cerevisiae encodes an α (1,2) mannosyl transferase required for branching the outer chain of N-linked oligosaccharides (Rayner J.C. and Munro S. 1998. J. Biol. Chem. 273: 26836–26843) and it also seems to have some effect on the transfer of mannosyl phosphate groups to the inner core (Olivero I. et al. 2000. FEBS Lett. 475: 111–116). In order to reveal possible interactions of MNN2 expression with other cellular pathways, we analyzed the transcriptome of the deletion mutant S. cerevisiae  mnn2Δ using cDNA microarrays. We found 151 genes that showed an altered expression level of ≥2-fold, 58 of them up-regulated and 93 down-regulated. Quite a high proportion of these genes (29%) encode unclassified proteins. In contrast to other defects affecting the integrity of the cell wall, deletion of MNN2 does not stimulate the expression of any of the genes included in the previously defined ‘cell wall compensatory cluster’ (Lagorce et al. 2003. J. Biol. Chem. 278: 20345–20357). We also found that 15% of the selected genes are related to central metabolic pathways. In addition, the mnn2Δ strain seems to have a certain level of stimulation of DNA processing reactions while some genes involved in intracellular transport pathways are under-regulated.


MNNCell wall DNA microarrays Mannoprotein Mannosyl phosphorylation N-glycosylation Saccharomyces cerevisiae 



cell wall integrity pathway


low dye binding


mannan defective


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Web pages

  1. EUROSCARF (European Saccharomyces cerevisiae archive for functional analysis): Scholar
  2. MIPS (Munich Information center for protein sequences): Scholar
  3. SGD (Saccharomyces cerevisiae genome database): Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Isaac Corbacho
    • 1
  • Isabel Olivero
    • 1
  • Stefan Hohmann
    • 2
  • Per Sunnerhagen
    • 2
  • Luis  M. Hernández
    • 1
  1. 1.Department of MicrobiologyUniversity of ExtremaduraBadajozSpain
  2. 2.Department of Cell and Molecular BiologyGöteborg University GöteborgSweden

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