Resuscitation-promoting factors: distribution among actinobacteria, synthesis during life-cycle and biological activity
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We have identified new members of the resuscitation-promoting factor (Rpf) protein family in numerous as yet unsequenced actinobacteria suggesting that Rpfs are common in this bacterial group. Formation and final delivery of the Rpf proteins varied from strain to strain and were dependent on growth conditions. Rpfs were found to act complementary to resuscitate other actinobacteria and might therefore be useful tools for the isolation of new actinobacterial species, especially from soil samples in which lots of microorganisms may have adopted dormant states.
Key wordsActinomycetes Non-culturable state Resuscitation-promoting factor
lactate minimal medium
galactose minimal medium
disodiumsuccinate minimal medium
transmembrane hidden Markov model (prediction of transmembrane helices in proteins)
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