A homozygous diploid subset of commercial wine yeast strains
Genetic analysis was performed on 45 commercial yeasts which are used in winemaking because of their superior fermentation properties. Genome sizes were estimated by propidium iodide fluorescence and flow cytometry. Forty strains had genome sizes consistent with their being diploid, while five had a range of aneuploid genome sizes that ranged from 1.2 to 1.8 times larger. The diploid strains are all Saccharomyces cerevisiae, based on genetic analysis of microsatellite and minisatellite markers and on DNA sequence analysis of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA of four strains. Four of the five aneuploid strains appeared to be interspecific hybrids between Saccharomyces kudriavzevii and Saccharomyces cerevisiae, with the fifth a hybrid between two S. cerevisiae strains. An identification fingerprint was constructed for the commercial yeast strains using 17 molecular markers. These included six published trinucleotide microsatellites, seven new dinucleotide microsatellites, and four published minisatellite markers. The markers provided unambiguous identification of the majority of strains; however, several had identical or similar patterns, and likely represent the same strain or mutants derived from it. The combined use of all 17 polymorphic loci allowed us to identify a set of eleven commercial wine yeast strains that appear to be genetically homozygous. These strains are presumed to have undergone inbreeding to maintain their homozygosity, a process referred to previously as ‘genome renewal’.
KeywordsFlow cytometry Internal transcribed spacer Microsatellite Minisatellite Polymorphism Saccharomyces
internal transcribed spacer
polymerase chain reaction
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- Antunovics Z., Nguyen H.-V., Gaillardin C. and Sipiczki M. 2005. Gradual genome stabilisation by progressive reduction of the Saccharomyces uvarum genome in an interspecific hybrid with Saccharomyces cerevisiae. FEMS Yeast Res. (in press) (epub ahead of print)Google Scholar
- Field D., Wills C., (1998). Abundant microsatellite polymorphism in Saccharomyces cerevisiae, and the different distributions of microsatellites in eight prokaryotes and S. cerevisiae, result from strong mutation pressures and a variety of selective forces. PNAS 95: 1647–1652PubMedCrossRefGoogle Scholar
- Giaever G., Chu A.M., Ni L., Connelly C., Riles L., Veronneau S., Dow S., Lucau-Danila A., Anderson K., Andre B., Arkin A.P., Astromoff A., El Bakkoury M., Bangham R., Benito R., Brachat S., Campanaro S., Curtiss M., Davis K., Deutschbauer A., Entian K.-D., Flaherty P., Foury F., Garfinkel D.J., Gerstein M., Gotte D., Guldener U., Hegemann J.H., Hempel S., Herman Z., Jaramillo D.F., Kelly D.E., Kelly S.L., Kotter P., LaBonte D., Lamb D.C., Lan N., Liang H., Liao H., Liu L., Luo C., Lussier M., Mao R., Menard P., Ooi S.L., Revuelta J.L., Roberts C.J., Rose M., Ross-Macdonald P., Scherens B., Schimmack G., Shafer B., Shoemaker D.D., Sookhai-Mahadeo S., Storms R.K., Strathern J.N., Valle G., Voet M., Volckaert G., Wang C.-y., Ward T.R., Wilhelmy J., Winzeler E.A., Yang Y., Yen G., Youngman E., Yu K., Bussey H., Boeke J.D., Snyder M., Philippsen P., Davis R.W., and Johnston M., (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature 418: 387–391PubMedCrossRefGoogle Scholar
- Henschke P.A. (2004). Yeast strains available for winemaking.2004/2005. Australian Wine Research Institute Technical Review 153: 8–24Google Scholar
- Legras J.-L., Ruh O., Merdinoglu D. and K.F. 2005. Selection of hypervariable microsatellite loci for the characterization of Sacchaormyces cerevisiae strains. Int. J. Food Microbiol. 102: 73–83Google Scholar
- Paulovich A.G. and Hartwell L.H. 1995. A checkpoint regulates the rate of progression through S phase in S. cerevisiae in response to DNA damage. Cell 82: 841–847Google Scholar
- Walker M.E., Gardner J.M., Vystavelova A., McBryde C., de Barros Lopes M., Jiranek V., (2003). Application of the reuseable, KanMX selectable marker to industrial yeast: construction and evaluation of heterothallic wine strains of Saccharomyces cerevisiae, possessing minimal foreign DNA sequences. FEMS Yeast Research 4: 339–347PubMedCrossRefGoogle Scholar
- White T.J., Brums T., Lee S., Taylor J., (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. San Diego, Academic PressGoogle Scholar
- Winston F., Dollard C. and Ricupero-Hovasse S.L. 1995. Construcion of a set convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast 11: 53–55Google Scholar