Antonie van Leeuwenhoek

, Volume 88, Issue 2, pp 173–179 | Cite as

BOX-PCR is an Adequate Tool for Typing Aeromonas spp.

  • Marta Tacão
  • Artur Alves
  • Maria José Saavedra
  • António Correia
Short Communication

Abstract

PCR-based methods of fingerprinting take advantage of the presence of repetitive sequences that are interspersed throughout the genome of diverse bacterial species. They include the repetitive extragenic palindromic (REP) sequence, the enterobacterial repetitive intergenic consensus sequence (ERIC) and the 154-bp BOX element. The combination of the three methods is used for fine discrimination of strains and is designated as rep-polymerase chain reaction (PCR). REP-PCR and ERIC-PCR have been shown to be useful for typing Aeromonas strains. To our knowledge, rep-PCR fingerprinting method using the BOXA1R primer has never been tested on aeromonads. In this study, the BOX-PCR fingerprinting technique was evaluated for the discrimination of strains of some Aeromonas species. All strains were typeable and the majority showed unique banding patterns. Four strains from culture collections were used to investigate the reproducibility of the method. According to our results, BOX-PCR fingerprinting is applicable for typing of Aeromonas strains and can be considered as a useful complementary tool for epidemiological studies of members of this genus.

Keywords

Aeromonas spp. BOX-PCR Molecular typing 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Marta Tacão
    • 1
  • Artur Alves
    • 1
  • Maria José Saavedra
    • 2
  • António Correia
    • 1
  1. 1.Department of Biology, Center for Cell BiologyUniversity of AveiroAveiroPortugal
  2. 2.CECAV – Department of Veterinary ScienceUniversity of Trás-os-Montes e Alto DouroVila RealPortugal

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