Antonie van Leeuwenhoek

, Volume 87, Issue 3, pp 195–203 | Cite as

Aspergillus vadensis, a new species of the group of black Aspergilli

  • Ronald P. de VriesEmail author
  • Jens C. Frisvad
  • Peter J. I. van de Vondervoort
  • Kim Burgers
  • Angelina F. A. Kuijpers
  • Robert A. Samson
  • Jaap Visser


A strain from the group of black Aspergilli was analysed in detail to determine the species to which it belongs. A detailed analysis of morphology, RFLP patterns and metabolite profiles was carried out. In addition, a phylogenetic tree was constructed for the black Aspergilli using the ITS and the β-tubulin sequences of the individual strains. The new species differs by its poor growth on glycerol and galacturonate and its unique extrolite profile consisting of aurasperone B, nigragillin, asperazine and kotanins. RFLP analysis using three genes as probes also resulted in a unique pattern. These data indicate that the strain was closely related but not identical to Aspergillus foetidus, Aspergillus niger and Aspergillus tubingensis. It was therefore designated as a novel species and named Aspergillus vadensis.


Aspergillus Metabolite analysis Morphology RFLP Sequence analysis 


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  1. Abarca, M.L., Accensi, F., Cano, J., Cabanes, F.J. 2004Taxonomy and significance of black aspergilli.Antonie van Leeuwenhoek863349PubMedGoogle Scholar
  2. Accensi, F., Cano, J., Figuera, L., Abarca, M.L., Cabanes, F.J. 1999New PCR method to differentiate species in the Aspergillus niger aggregateFEMS Microb. Lett.15191196Google Scholar
  3. Al-Musallam A. 1980. Revision of the black Aspergillus species. PhD thesis, Utrecht University, The Netherlands.Google Scholar
  4. Bos, C.J., Debets, A.J.M., Swart, K., Huybers, A., Kobus, G., Slakhorst, S.M. 1988Genetic analysis and the construction of master strains for assignment of genes to six linkage groups in Aspergillus niger Curr. Genet.14437443PubMedGoogle Scholar
  5. de Hoog, G.S., Gerrits-van den Ende, A.H.G. 1998Molecular diagnostics of clinical strains of filamentous basidiomycetesMycoses41183189PubMedCrossRefGoogle Scholar
  6. de Vries, R.P., Burgers, K., van deVondervoort, P.J.I., Frisvad, J.C., Samson, R.A., Visser, J. 2004A new black Aspergillus species, A. vadensisis a promising host for homologous and heterologous protein productionAppl. Environ. Microbiol.7039543959PubMedGoogle Scholar
  7. de Vries R.P., vande Vondervoort P.J.I. and Visser J. 2002. Method for the production of proteins and polypeptides using fungal cells, in particular Aspergillus vadensis. Patent WO 02/02825 A1. Google Scholar
  8. Frisvad, J.C. 1987High-performance liquid chromatographic determination of profiles of mycotoxins and other secondary metabolitesJ. Chromatogr.392333347PubMedGoogle Scholar
  9. Frisvad, J.C., Filtenborg, O., Thrane, U. 1989Analysis and screening for mycotoxins and other secondary metabolites in fungal cultures by thin-layer chromatography and high-performance liquid chromatographyArch. Environ. Contam. Toxicol.18331335PubMedGoogle Scholar
  10. Frisvad, J.C., Thrane, U. 1987Standardized high-performance liquid chromatography of 182 mycotoxins and other fungal metabolites based on alkylphenone retention indices and UV-VIS spectra (diode array detection)J. Chromatogr.404195214PubMedGoogle Scholar
  11. Glass, N.L., Donaldson, G.C. 1995Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetesAppl. Environ. Microbiol.6113231330PubMedGoogle Scholar
  12. Hiort , J. eds. 2003Neue Naturstoffe aus Schwamm-assozierten Pilzen des Mittelmeeres. PhD thesisMathematisch-Naturwissenschaftlichen FakultätHeinrich-Heine-UniversitätDusseldorfGermanyGoogle Scholar
  13. Kusters-van Someren, M.A., Samson, R.A., Visser, J. 1991The use of RFLP analysis in classification of the black AspergilliReinterpretation of the Aspergillus niger aggregateCurr. Genet.192126Google Scholar
  14. Masclaux, F., Guhc)ho, E., de Hoog, G.S., Christen, R. 1995Phylogenetic relationships of human pathogenic Cladosporium (Xylohypha) species inferred from partial LS rRNA sequencesJ. Med. Vet. Mycol.33327338PubMedGoogle Scholar
  15. Parenicová, L., Benen, J.A.E., Samson, R.A., Visser, J. 1997Evaluation of RFLP analysis of the classification of selected black aspergilliMycol. Res.101810814Google Scholar
  16. Parenicová, L., Skouboe, P., Frisvad, J., Samson, R.A., Rossen, L., ten Hoor-Suykerbuyk, M., Visser, J. 2001Combined molecular and biochemical approach identifies Aspergillus japonicus Aspergillus aculeatus as two speciesAppl. Environ. Microbiol.67521527PubMedGoogle Scholar
  17. Parenicová, L., Suykerbuyk, M.E.G., Samson, R.A., Visser, J. 1996Evaluation of restriction fragment length polymorphism for the classification of Aspergillus carbonarius African J. Mycol. Biotechnol.41319Google Scholar
  18. Peterson, S.W., Ito, Y., Horn, B.W., Goto, T. 2001Aspergillus bombycisa new aflatoxigenic species and genetic variation in its sibling species, A. nomiusMycologia93689703Google Scholar
  19. Raper, K.B., Fennell, D.I. 1965The genus AspergillusWilliams & Williams CompanyBaltimoreGoogle Scholar
  20. Sambrook, J., Fritsch, E.F., Maniatis, T. 1989Molecular cloning a laboratory manualCold Spring Harbour LaboratoryCold Spring HarbourN.YGoogle Scholar
  21. Samson, R.A. 1994Current systematics of the genus AspergillusPowell, K.A.Renwick, A.Peberdy, J.F. eds. The genus Aspergillus: From taxonomy and genetics to industrial applicationPlenum PressLondonGoogle Scholar
  22. Smedsgaard, J. 1997Micro-scale extraction procedure for standardized screening of fungal metabolite production in culturesJ. Chromatogr. A760264270PubMedGoogle Scholar
  23. Swofford, D.L. 2000PAUP* 4.0: phylogenetic analysis using parsimonySinauer AssociatesSunderlandMAGoogle Scholar
  24. Varga, J., Kevei, F., Fekete, C., Coenen, A., Kozakiewicz, Z., Croft, J.H. 1993Restriction fragment length polymorphisms in the mitochondrial DNAs of the Aspergillus niger aggregateMycol. Res.9712071202CrossRefGoogle Scholar
  25. Varga, J., Kevei, F., Vriesema, A., Debets, F., Kozakiewicz, Z., Croft, J.H. 1994Mitochondrial DNA restriction fragment length polymorphisms in field isolates of the Aspergillus niger aggregateCan. J. Microbiol.40612621PubMedCrossRefGoogle Scholar
  26. Vishniac, W., Santer, M. 1957The thiobacilliBacteriol. Rev.21195213PubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Ronald P. de Vries
    • 1
    • 2
    Email author
  • Jens C. Frisvad
    • 3
  • Peter J. I. van de Vondervoort
    • 1
    • 5
  • Kim Burgers
    • 1
    • 6
  • Angelina F. A. Kuijpers
    • 4
  • Robert A. Samson
    • 4
  • Jaap Visser
    • 1
    • 7
  1. 1.Molecular Genetics of Industrial MicroorganismsWageningen UniversityWageningenThe Netherlands
  2. 2.MicrobiologyUtrecht UniversityUtrechtThe Netherlands
  3. 3.Mycology GroupTechnical University of DenmarkLyngbyDenmark
  4. 4.Centraalbureau voor SchimmelculturesUtrechtThe Netherlands
  5. 5.Laboratory of PhytopathologyWageningen UniversityWageningenThe Netherlands
  6. 6. CatchMabs WageningenThe Netherlands
  7. 7.Fungal Genetics and Technology ConsultancyWageningenThe Netherlands

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