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Agroforestry Systems

, Volume 92, Issue 2, pp 525–540 | Cite as

Effect of tree-enset-coffee based agro-forestry practices on arbuscular mycorrhizal fungi (AMF) species diversity and spore density

  • Beyene DoboEmail author
  • Fassil Asefa
  • Zebene Asfaw
Article

Abstract

A study on arbuscular mycorrhizal fungal (AMF) spore abundance and species composition was undertaken along different tree-enset-coffee based agroforestry practices in Sidama administrative region of Southern Ethiopia. Soil samples were collected from 36 sampling points for each tree-enset-coffee combinations within the nine sampling sites. The soil samples were processed for soil physicochemical properties; AMF spore abundance, AMF species composition and soil trap cultures. In tree-enset-coffee based agro-forestry practices there was significant (P < 0.05) effects on AMF spore abundance among different combinations. However, land uses with Millettia ferruginea and Erythrina brucei had higher spore numbers than land use types with Cordia africana as a shade tree. AMF species also seemed to show preference for specific tree-crop combination. Percentage root colonization of five months old Sorghum bicolor grown as a trap plant in field soils collected along the tree-crop combinations from the experimental sites showed significantly different (P < 0.05) root colonization. There was a positive correlation (r = 0.458, P = 0.016) between root colonization and spore abundance at the 0.05 level (2-tailed). A total of 28 AMF morphotypes from eight genera were isolated. Species diversity also responded differently to different tree-crop combinations, in tree-enset-coffee based agroforestry practices for all experimental sites. The response of AMF species to different tree-crop combinations therefore indicates the importance of selection of specific species of trees for better management of small holder at low external input agroforestry practices.

Keywords

Arbuscular mycorrhizae fungi Tree-enset-coffee Gradients Agroforestry practice 

Notes

Acknowledgements

We would like to acknowledge Hawassa College of Teacher Education for financial and logistic supports; Department of Microbiology, Cellular & Molecular Biology, Addis Ababa University and college of agriculture, Hawassa University for their support with chemicals and laboratory equipments. Finally we would like to thank Vestberg Mourith (PhD) & Zerihun Belay (PhD) for their unreserved comments on taxonomic positions of AMF spores.

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Department of Natural Resources Management and Environmental SciencesHaramaya UniversityHaramayaEthiopia
  2. 2.Department of Microbial, Cellular and Molecular Biology, College of Natural SciencesAddis Ababa UniversityAddis AbabaEthiopia
  3. 3.Wondo Genet College of Forestry and Natural ResourcesHawassa UniversityHawassaEthiopia

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