Agroforestry Systems

, 83:149 | Cite as

Ecophysiological responses of three native herbs to phytotoxic potential of invasive Acacia melanoxylon R. Br.

  • M. Iftikhar Hussain
  • L. González
  • C. Souto
  • M. J. Reigosa
Article

Abstract

Acacia melanoxylon R. Br. (Blackwood) is a native Australian species that has invaded woodlands and degraded natural habitats in the north western Iberian Peninsula (Galicia), Spain. Several phenolic (p-hydroxybenzoic, vanillic, p-coumaric, syringic, protocatequic, ferulic acids) and flavonoids (catechin, luteolin, rutin, apigenin, and quercetin) were identified from methanol extracts of flowers and phyllodes of A. melanoxylon by HPLC. Flowers and phyllodes of A. melanoxylon were soaked separately in the water in a ratio of 1:1 (w/v) for 24 h to prepare aqueous extracts (100, 75, 50 and 25%) and distilled water was used as control. The seeds of three native plants, cocksfoot (Dactylis glomerata), perennial ryegrass (Lolium perenne), common sorrel (Rumex acetosa) and a general test crop lettuce (Lactuca sativa) were grown in perlite culture and aqueous extracts of A. melanoxylon (flowers and phyllodes) were applied exogenously at various concentrations. A. melanoxylon flowers extract (100%) inhibited the shoot length of D. glomerata and L. perenne by 31 and 20% of the control, respectively. Leaf and root fresh weights of L. perenne, D. glomerata, and L. sativa were reduced after treatment with acacia flowers and phyllodes extract. Leaf relative water content of D. glomerata and L. perenne was reduced by acacia flowers and phyllodes extract at all concentrations. Both extracts reduced leaf osmotic potential in D. glomerata, L. perenne and L. sativa. Quantum efficiency of open PSII reaction centers (Fv/Fm) and quantum yield (ΦPSII) of photosystem II were decreased in L. perenne, D. glomerata; R. acetosa and L. sativa after treatment with acacia flowers/phyllodes extract at 100% concentration. Acacia flowers and phyllodes extract (100%) inhibited the qP level during all 6 days in D. glomerata, L. perenne, R. acetosa and L. sativa. A significant reduction in NPQ was observed during different days in all four plant species due to Acacia flowers and phyllodes extract (100%). The δ13C ratios were less negative in L. perenne, D. glomerata and L. sativa as compared to the control. A. melanoxylon flowers and phyllodes extract (100%) significantly reduced leaf protein contents in D. glomerata, L. perenne and in L. sativa.

Keywords

Allelopathy Phenolics Acacia melanoxylon Dactylis glomerata Lolium perenne Carbon isotopes discrimination Rumex acetosa Flavonoids 

List of symbols

C%

Carbon concentration

ci/ca

Ratio of intercellular CO2 concentration from leaf to air

\( {\text{F}}^{\prime}_{\text{m}} \)

Maximal fluorescence level from light-adapted leaves

\( {\text{F}}^{\prime}_{0} \)

Minimal fluorescence level from light-adapted leaves

\( {\text{F}}^{\prime}_{\text{v}} \)

Variable fluorescence level from light-adapted state

Fm

Maximal fluorescence level from dark-adapted leaves

F0

Initial fluorescence level from dark-adapted leaves

Fv

Variable fluorescence level from dark-adapted leaves

Fv/Fm

Efficiency of photosystem II photochemistry in the dark-adapted state

LOP

Leaf osmotic potential

NPQ

Non-photochemical quenching

qP

Photochemical quenching

RWC

Relative water content

δ13C

Composition of carbon isotope ratios

Δ13C

Carbon isotope discrimination

ΦPSII

Maximum quantum yield of PSII electron transport

Notes

Acknowledgments

We thank Dr. Aldo Barreiro, Carlos Bolaño, Alfredo Justo, Maite Ricart, and P. Lorenzo for field and laboratory assistance. We are also grateful to Jesús Estévez Sío and Jorge Millos for their technical assistance with isotope ratio mass spectroscopy. We also thank Dr. Nuria Pedrol for help in osmotic potential measurement and Dr. Adela Sánchez for useful discussion. We are extremely thankful to Dr. Jose Shibu (Editor) and advisors for their valuable comments for the improvement of the article.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • M. Iftikhar Hussain
    • 1
  • L. González
    • 1
  • C. Souto
    • 2
  • M. J. Reigosa
    • 1
  1. 1.Department of Plant Biology and Soil ScienceUniversity of VigoVigoSpain
  2. 2.Plant Production SectionE.U.E.T. ForestryPontevedraSpain

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