Agroforestry Systems

, Volume 78, Issue 2, pp 139–150

Influence of improved fallow systems and phosphorus application on arbuscular mycorrhizal fungi symbiosis in maize grown in western Kenya

  • Mary Nyawira Muchane
  • Bashir Jama
  • Caleb Othieno
  • Robert Okalebo
  • David Odee
  • Joseph Machua
  • Jan Jansa


A field study was carried out on a six-year-old on-farm field trial during long-rains season (April–August) 2003 to investigate the effect of improved fallow systems and phosphorus application on arbuscular mycorrhiza fungi (AMF) symbiosis in maize. The trial comprised of maize rotated with a fast growing leguminous Crotalariagrahamiana fallow and a non-leguminous Tithonia diversifolia fallow for 3 years followed by continuous maize. The experiment was randomized complete block design with three cropping (continuous maize, Crotalaria fallow and Tithonia fallow) systems and two phosphorus levels (0 and 50 kg P/ha). AMF colonization in maize roots, maize yield and macro-nutrients uptake were recorded. Phosphorus applications improved (P < 0.05) early (<8 weeks old maize) AMF colonization, nutrient uptake and maize yield in improved fallow systems. Greater differences due to phosphorus application were noted in maize in Tithonia fallow than in Crotalaria fallow. Following phosphorus application, a positive relationship existed between early AMF colonization and maize yield (r = 0.38), and phosphorus and nitrogen uptake (r = 0.40 and r = 0.43, respectively), demonstrating the importance of phosphorus fertilization in enhancing low-input technologies (improved fallows systems) in phosphorus deficient and acidic soils of western Kenya.


Arbuscular mycorrhiza fungi Crotalaria fallow Continuous maize Phosphorus Tithonia fallow 


  1. Allison VJ, Goldberg DE (2002) Species-level versus community-level patterns of mycorrhizal dependence on phosphorus: an example of Simpson’s paradox. Funct Ecol 16:346–352CrossRefGoogle Scholar
  2. Bolan NS, Robson AD, Barrow NJ (1984) Increasing phosphorus supply can increase the infection of plant roots by vesicular-arbuscular mycorrhizal fungi. Soil Biol Biochem 16:419–420CrossRefGoogle Scholar
  3. Borie F, Redel Y, Rubio R, Rouanet (2002) Interactions between crop residues application and mycorrhizal developments and some soil-root interface properties and mineral acquisition by plants in an acidic soil. Biol Fertil Soils 36:151–160CrossRefGoogle Scholar
  4. Buerkert A, Bationo A, Piepho HP (2001) Efficient phosphorus application strategies for increased crop production in sub-Saharan West Africa. Field Crops Res 72:1–15CrossRefGoogle Scholar
  5. Bünemann EK, Bossio DA, Smithson PC, Frossard E, Oberson A (2004a) Microbial community composition and substrate use in a highly weathered soil as affected by crop rotation and P fertilization. Soil Biol Biochem 36:889–901CrossRefGoogle Scholar
  6. Bünemann EK, Smithson PC, Jama B, Frossard E, Oberson A (2004b) Maize productivity and nutrient dynamics in maize-fallow rotations in western Kenya. Plant Soil 264:195–208CrossRefGoogle Scholar
  7. Bünemann EK, Steinebrunner F, Smithson PC, Frossard E, Oberson A (2004c) Phosphorus dynamics in a highly weathered soil as revealed by isotopic labeling techniques. Soil Sci Soc Am J 68:1645–1655Google Scholar
  8. Buresh RJ (1999) Agroforestry strategies for increasing the efficiency of phosphorus use in tropical uplands. Agrofor Forum 9(4):8–13Google Scholar
  9. Cardoso IM, Kuyper TW (2006) Mycorrhizas and tropical soil fertility. Agric Ecosyst Environ 116:72–84CrossRefGoogle Scholar
  10. Cardoso IM, Boddington C, Janssen BH, Oenema O, Kuyper TW (2006) Differential access to phosphorus pools of an oxisol by mycorrhizal and non-mycorrhizal maize. Commun Soil Sci Plant Anal 37:1–15CrossRefGoogle Scholar
  11. Evans DG, Miller MH (1998) Vesicular-arbuscular mycorrhizas and the soil-induced reduction of nutrient absorption in maize. I. Causal relations. New Phytol 110:67–74CrossRefGoogle Scholar
  12. García IV, Mendoza RE (2007) Arbuscular mycorrhizal fungi and plant symbiosis in a saline-sodic soil. Mycorrhiza 17:167–174CrossRefPubMedGoogle Scholar
  13. Gathumbi SM, Cadisch G, Giller KE (2002) 15N natural abundance as a tool for assessing N2-fixation of herbaceous, shrub and tree legumes in improved fallows. Soil Biol Biochem 34:1059–1071CrossRefGoogle Scholar
  14. Gavito ME, Miller MH (1998) Early phosphorus nutrition, mycorrhizae development, dry matter and partitioning and yield of maize. Plant Soil 199:177–186CrossRefGoogle Scholar
  15. George E, Marschner H, Jakobsen I (1995) Role of arbuscular mycorrhizal fungi in uptake of phosphorus and nitrogen from soil. Crit Rev Biotechnol 15:257–270CrossRefGoogle Scholar
  16. Gichuru MP, Bationo A, Bekundu MA, Goma HC, Mafongonya PL, Mugadi D, Murwira H, Nendwa SM, Nyathi P, Swift MJ (2003) Soil fertility management in Africa: regional perspective. Academy Science publishers, Nairobi, 322 ppGoogle Scholar
  17. Harteminkv AE, Buresh RJ, Jama B, Janssen BH (1996) Soil nitrate and water dynamics in Sesbania fallows, weed fallows, and maize. Soil Sci Soc Am J 60:568–574CrossRefGoogle Scholar
  18. ICRAF (2000) Laboratory working manual. Plant and soil routine analysis of elements. World Agroforestry Centre, NairobiGoogle Scholar
  19. Jaetzold R, Schmidt H (1982) Farm management handbook of Kenya, vol II. Natural conditions and farm management information. Part A: West Kenya. Ministry of Agriculture, Kenya, in cooperation with the German Agricultural Team (GAT) of the German Agency for Technical Cooperation (GTZ), Kenya, 397 ppGoogle Scholar
  20. Jama B, Swinkels RA, Buresh RJ (1997) Agronomic and economic evaluation of organic and inorganic sources of phosphorus in Western Kenya. Agron J 89(4):597–604CrossRefGoogle Scholar
  21. Kahiluoto H, Ketoja E, Vestberg M, Saarela I (2001) Promotion of AM utilization through reduced P fertilization 2. Field studies. Plant Soil 231:65–79CrossRefGoogle Scholar
  22. Karasawa T, Arihara J, Kasahara Y (2000) Effects of previous cropping on arbuscular mycorrhizal formation on growth of maize under various soil moisture conditions. Soil Sci Plant Nutr 46:53–60Google Scholar
  23. Karasawa T, Kasahara Y, Takebe M (2001) Variable response of growth and arbuscular mycorrhizal colonization of maize plants to preceding crops in various types of soil. Biol Fertil Soils 33:286–293CrossRefGoogle Scholar
  24. Koide RT (2000) Functional complementarity in the arbuscular mycorrhizal symbiosis. New Phytol 147:233–235CrossRefGoogle Scholar
  25. Li H, Smith SE, Holloway RE, Zhu Y, Smith FA (2006) Arbuscular mycorrhizal fungi contribute to phosphorus uptake by wheat grown in a phosphorus-fixing soil even in the absence of positive growth responses. New Phytol 172:536–543CrossRefPubMedGoogle Scholar
  26. Maroko JB, Buresh RJ, Smithson PC (1999) Soil phosphorus Institute, in unfertilized fallow-maize systems on two tropical soils. Soil Sci Soc Am J 63:320–326Google Scholar
  27. Mason P, Ingleby K (1998) Mycorrhizae working manual. ITP, ScotlandGoogle Scholar
  28. Mathimaran N, Ruh R, Jama B, Verchot L, Frossard E, Jansa J (2007) Impact of agricultural management on arbuscular mycorrhizal fungal communities in Kenyan ferralsol. Agric Ecosyst Environ 119:22–32CrossRefGoogle Scholar
  29. McGonigle TP, Evans DG, Miller MH, Fairchild GL, Swan JA (1990) A new method which gives an objective measure of colonization and phosphorus absorption by maize in growth chamber and field experiments. New Phytol 116:629–636CrossRefGoogle Scholar
  30. Mekonnen K, Buresh RJ, Jama B (1997) Root and inorganic nitrogen distributions in Sesbania fallow, natural fallow and maize fields. Plant Soil 188:319–327CrossRefGoogle Scholar
  31. Mendoza RE, Pagani E (1997) Influence of phosphorus nutrition on mycorrhizal growth response and morphology of mycorrhizae in Lotus tenuis. J Plant Nutr 20:625–639CrossRefGoogle Scholar
  32. Miller MH (2000) Arbuscular mycorrhizae and the phosphorus nutrition of maize: a review of Guelph studies. Can J Plant Sci 80:47–52Google Scholar
  33. Miller M, Mcgonigle T, Addy HP (1995) Functional ecology of vesicular arbuscular mycorrhizas as influenced by phosphate fertilization and tillage in agricultural ecosystems. Crit Rev Biotechonol 15:241–255CrossRefGoogle Scholar
  34. Morton JB (1988) Taxonomy of VA mycorrhizal fungi: classification, nomenclature, and identification. Mycotaxonomy 32:267–324Google Scholar
  35. Nziguheba G, Palm CA, Buresh RJ, Smithson PC (1998) Soil phosphorus fractions and sorption as affected by organic and inorganic sources. Plant Soil 198:159–168CrossRefGoogle Scholar
  36. Okalebo JR, Gathua KW, Woomer PL (2002) Laboratory methods of soil and plant analysis working manual, 2nd edn. TSBF, NairobiGoogle Scholar
  37. Picone C (2002) Managing mycorrhizae for sustainable agriculture in the tropics. In: Vandermeer JH (ed) Tropical agroecosystems. CRC, Boca Raton, pp 95–132Google Scholar
  38. Sanchez PA (1999) Improved fallows come of age in the tropics. Agrofor Syst 47:3–12CrossRefGoogle Scholar
  39. Sanchez PA, Shepherd KD, Soule MJ, Place FM, Mukwunye AU, Bursch RJ, Kwesiga FR, Izac A-MN, Ndiritu CG, Woomer PL (1997) Soil fertility replenishment in Africa: an investment in natural resource capital. In: Bursch RJ, Sanchez PA, Calhoon F (eds) Replenishing soil fertility in Africa. Soil science society of America special publication No. 51. Madison, WI, pp 1–46Google Scholar
  40. Shepherd KD, Ohlsson E, Okalebo JR, Ndufa JK (1996) Potential impact of agroforestry on soil nutrient balance at the farm scales in East Africa Highland. Fertil Resour 44:87–99CrossRefGoogle Scholar
  41. Sieverding E (1990) Ecology of VAM fungi in tropical agrosystems. Agric Ecosyst Environ 29:369–390CrossRefGoogle Scholar
  42. Singer JW, Cox WJ (1998) Corn growth and yield under different rotation, tillage and management systems. Crop Sci 38:996–1003Google Scholar
  43. Smithson PC, Giller KE (2002) Appropriate farm management practices for alleviating N and P deficiencies in low-nutrient soils of the tropics. Plant Soil 245:169–180CrossRefGoogle Scholar
  44. Thompson JP (1991) Improving the mycorrhizal condition of the soil through cultural practices and effects on growth and phosphorus uptake by plants. In: Johansen (ed) Phosphorus nutrition of grain legumes in the semi-arid tropics. ICRISAT, Patancheru, pp 117–138Google Scholar
  45. Thompson JP (1994) What is the potential for management of mycorrhizas in agriculture? In: Robison AD, Abbott LK, Malaczuk N (eds) Management of mycorrhizae in agriculture, horticulture and forestry. Kluwer, The Netherlands, pp 191–200Google Scholar
  46. Treseder KK, Allen MF (2002) Direct N and P limitation of arbuscular mycorrhizal fungi: a model and field test. New Phytol 155:507–515CrossRefGoogle Scholar
  47. Trouvelot A, Kough JL, Gianinazzi-Pearson (1986) Mesure du taux de mycorhization VA d’un syste’me radivulaire. Recherche de me’thodes d’estimation ayant une significantion fonctionnelle. In: Gianinazzi-Pearson V, Gianinazzi S (eds) Physiological and genetic aspects of mycorrhizae. INRA, Paris, pp 217–221Google Scholar
  48. Weng L, Van Riemsdijk WH, Hiemstra T (2008) Humic nanoparticles at the oxide–water interface: interactions with phosphate ion adsorption. Environ Sci Technol 42(23):8747–8752CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Mary Nyawira Muchane
    • 1
  • Bashir Jama
    • 2
  • Caleb Othieno
    • 5
  • Robert Okalebo
    • 5
  • David Odee
    • 3
  • Joseph Machua
    • 3
  • Jan Jansa
    • 4
  1. 1.Botany DepartmentNational Museums of KenyaNairobiKenya
  2. 2.World Agroforestry Centre (ICRAF)NairobiKenya
  3. 3.Kenya Forestry Research Institute (KEFRI)NairobiKenya
  4. 4.Swiss Federal Institute of Technology (ETH) ZurichLindauSwitzerland
  5. 5.Department of Soil ScienceMoi UniversityEldoretKenya

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