Somatic NRAS mutation in patient with generalized lymphatic anomaly
- 498 Downloads
Generalized lymphatic anomaly (GLA or lymphangiomatosis) is a rare disease characterized by a diffuse proliferation of lymphatic vessels in skin and internal organs. It often leads to progressive respiratory failure and death, but its etiology is unknown. Here, we isolated lymphangiomatosis endothelial cells from GLA tissue. These cells were characterized by high proliferation and survival rates, but displayed impaired capacities for migration and tube formation. We employed whole exome sequencing to search for disease-causing genes and identified a somatic mutation in NRAS. We used mouse and zebrafish model systems to initially evaluate the role of this mutation in the development of the lymphatic system, and we studied the effect of drugs blocking the downstream effectors, mTOR and ERK, on this disease.
KeywordsLymphangiomatosis NRAS Mutation
Lymphatic endothelial cells
Human dermal lymphatic endothelial cells
Lymphangiomatosis endothelial cells
Mammalian target of rapamycin
Vascular endothelial growth factor A
Whole exome sequencing
We thank M. Grunspan for help with zebrafish experiments; Sarit Farage-Barhom for her help with bioinformatics analysis; Michael Dellinger, The Lymphatic Malformation Institute (LMI) for fruitful discussions. This project was supported by grants from the Israel Science Foundation (Grant No. 1716/11 to S.G.), Basil O’Connor Starter Scholar Research Award Grant from the March of Dimes (No. 5-FY12-55, to S.G), Marie Curie Re-integration grant (FP7-PEOPLE-2010-IRG to S.G).
E.M., G.L., N.A., A.B.,G.R., K.Y., and S.G. designed research studies; E.M., G.L., O.B., I.D., L.S., E.E., A.H., and K.C. conducted experiments and acquired data; E.M., G.L., O.B., I.D., L.S., E.E., I.P., and U.R., A.B., G.R., K.Y., and S.G analyzed and interpreted data; I.P, and U.R. provided human tissue samples; E.M., G.L., G.R., K.Y., and S.G wrote the manuscript.
Compliance with ethical standards
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.
- 4.Kadakia KC, Patel SM, Yi ES, Limper AH (2013) Diffuse pulmonary lymphangiomatosis. Can Resp J J Can Thorac Soc 20:52–54Google Scholar
- 11.DeCicco-Skinner KL, Henry GH, Cataisson C et al (2014) Endothelial cell tube formation assay for the in vitro study of angiogenesis. J Vis Exp 91:e51312Google Scholar
- 17.Greenberger S, Yuan S, Walsh LA, Boscolo E, Kang KT, Matthews B, Mulliken JB, Bischoff J (2011) Rapamycin suppresses self-renewal and vasculogenic potential of stem cells isolated from infantile hemangioma. J Invest Dermatol 131:2467–2476Google Scholar
- 44.Lokmic Z, Mitchell GM, Koh Wee Chong N, Bastiaanse J, Gerrand YW, Zeng Y, Williams ED, Penington AJ (2014) Isolation of human lymphatic malformation endothelial cells, their in vitro characterization and in vivo survival in a mouse xenograft model. Angiogenesis 17(1):1–15. https://doi.org/10.1007/s10456-013-9371-8