Angiogenesis

, Volume 17, Issue 4, pp 779–804 | Cite as

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

Review Paper

Abstract

The chicken chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development, including but not restricted to gas exchange. Over the last two decades, interest in the CAM as a robust experimental platform to study blood vessels has been shared by specialists working in bioengineering, development, morphology, biochemistry, transplant biology, cancer research and drug development. The tissue composition and accessibility of the CAM for experimental manipulation, makes it an attractive preclinical in vivo model for drug screening and/or for studies of vascular growth. In this article we provide a detailed review of the use of the CAM to study vascular biology and response of blood vessels to a variety of agonists. We also present distinct cultivation protocols discussing their advantages and limitations and provide a summarized update on the use of the CAM in vascular imaging, drug delivery, pharmacokinetics and toxicology.

Keywords

Angiogenesis Anti-vascular therapies Chicken chorioallantoic membrane Bioengineering Lymphangiogenesis Organ transplantation Physical forces Tumor vasculature 

Abbreviations

AA

Anti-angiogenic

AC

Anti-cancer

ALA

Aminolevulinic acid

AM

Anti-microbial

AMD

Age-related macular degeneration

BrdU

Bromodeoxyuridine

CAM

Chicken chorioallantoic membrane

CNV

Subfoveal choroidal neovascularization

DDS

Drug delivery systems

DPPC

Dipalmitoylphosphatidylcholine

ECs

Endothelial cells

ECM

Extracellular matrix

GF

Growth factor

HGF

Hepatocyte growth factor

LC

Langerhans cells

LF

Lipid factor

MR

Microbeam radiation

PCV

Polypoidal choroidal vasculopathy

PDD

Photodynamic diagnosis

PEG

Polyethylene glycol

PLGA

Poly(lactide-co-glycolide)

PpIX

Protoporphyrin IX

Prox1

Prospero homeobox protein 1

PS

Photosensitizer

SMA

Smooth muscle actin

VDA

Vascular disrupting agents

VM

Vasculogenic mimicry

YSM

Yolk sac membrane

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Institute of Chemical Sciences and EngineeringSwiss Federal Institute of Technology (EPFL)LausanneSwitzerland
  2. 2.Department of Chemical and Biomolecular EngineeringUCLALos AngelesUSA
  3. 3.Molecular Biology InstituteUCLALos AngelesUSA

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