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Angiogenesis

, Volume 16, Issue 3, pp 553–560 | Cite as

Reduction of connexin43 in human endothelial progenitor cells impairs the angiogenic potential

  • Hsueh-Hsiao Wang
  • Cheng-Huang Su
  • Yih-Jer Wu
  • Jiun-Yi Li
  • Ya-Ming Tseng
  • Yi-Chun Lin
  • Chin-Ling Hsieh
  • Cheng-Ho Tsai
  • Hung-I YehEmail author
Original Paper

Abstract

Our previous work showed that arsenic trioxide down-regulated Cx43 and attenuated the angiogenic potential of human late endothelial progenitor cells (EPC). However, the relation between Cx43 and angiogenic activity of the EPC remained unclear. In the study, human late EPC were treated with siRNA specific to Cx43 (Cx43siRNA). The expression profiles as well as activity of the treated cells were examined. In parallel, the angiogenic potential of human EPC treated with Cx43siRNA was evaluated using murine hind limb ischemic model. The results showed that, in the EPC treated with Cx43siRNA, the activity of migration, proliferation, and angiogenic potential were attenuated, accompanied by reduction in vascular endothelial growth factor (VEGF) expression. In hind limb ischemia mice, EPC treated with Cx43siRNA lost the therapeutic angiogenic potential. VEGF supplementation partially recovered the activity impaired by Cx43 down-regulation. In conclusion, reduced Cx43 expression per se in the EPC causes decreased expression of VEGF and impaired angiogenic potential of the cells. Prevention of Cx43 reduction is a potential target to maintain the angiogenic potential of the EPC.

Keywords

Angiogenesis Connexin43 Connexins Endothelial progenitor cell 

Notes

Acknowledgments

We gratefully acknowledge the supports of research grants from Taiwan (R.O.C.): National Science Council (NSC 99-2314-B-195-009-MY2) and Mackay Memorial Hospital (MMH-E-99003, 100-03).

Conflict of interest

None.

Supplementary material

10456_2013_9335_MOESM1_ESM.doc (3.2 mb)
Supplementary material 1 (DOC 3240 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Hsueh-Hsiao Wang
    • 1
    • 2
  • Cheng-Huang Su
    • 2
    • 3
    • 5
  • Yih-Jer Wu
    • 1
    • 3
  • Jiun-Yi Li
    • 4
  • Ya-Ming Tseng
    • 1
  • Yi-Chun Lin
    • 2
  • Chin-Ling Hsieh
    • 2
  • Cheng-Ho Tsai
    • 1
    • 3
  • Hung-I Yeh
    • 1
    • 3
    Email author
  1. 1.Department of MedicineMackay Medical CollegeSanzhi District, New Taipei CityTaiwan
  2. 2.Department of Medical ResearchMackay Memorial HospitalTaipeiTaiwan
  3. 3.Department of Internal MedicineMackay Memorial HospitalTaipeiTaiwan
  4. 4.Department of SurgeryMackay Memorial HospitalTaipeiTaiwan
  5. 5.Mackay Medicine, Nursing and Management CollegeTaipeiTaiwan

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