, Volume 15, Issue 1, pp 87–98 | Cite as

In vivo laser speckle imaging reveals microvascular remodeling and hemodynamic changes during wound healing angiogenesis

  • Abhishek Rege
  • Nitish V. Thakor
  • Kevin Rhie
  • Arvind P. PathakEmail author
Original Paper


Laser speckle contrast imaging (LSCI) is a high-resolution and high contrast optical imaging technique often used to characterize hemodynamic changes in short-term physiological experiments. In this study, we demonstrate the utility of LSCI for characterizing microvascular remodeling and hemodynamic changes during wound healing angiogenesis in vivo. A 2 mm diameter hole was made in the mouse ear and the periphery of the wound imaged in vivo using LSCI over 12 days. We were able to visualize and quantify the vascular and perfusion changes that accompanied wound healing in the microenvironment proximal to the wound, and validated these changes with histology. We found that consistent with the stages of wound healing, microvessel density increased during the initial inflammatory phase (i.e., day 0–3), stayed elevated through the tissue formation phase (i.e., until day 7) and returned to baseline during the tissue remodeling phase (i.e., by day 12). Concomitant “wide area mapping” of blood flow revealed that tissue perfusion in the wound periphery initially decreased, gradually increased from day 3–7, and subsided as healing completed. Interestingly, some regions exhibited a reestablishment of tissue perfusion approximately 6 days earlier than the ~18 days usually reported for the long term remodeling phase. The results from this study demonstrate that LSCI is an ideal platform for elucidating in vivo changes in microvascular hemodynamics and angiogenesis, and has the potential to offer invaluable insights in a range of disease models involving abnormal hemodynamics, such as diabetes and tumors.


Angiogenesis Hemodynamics Laser speckle imaging Wound healing Vasculature 



This work was supported jointly by National Institute of Aging award number R01AG029681, Department of Health and Human Services award number 1R43CA139983-01, and a JHU Institute of NanoBiotechnology (INBT) Junior Faculty Pilot Award. We gratefully acknowledge Peng Miao for assistance with image registration.

Supplementary material

10456_2011_9245_MOESM1_ESM.ppt (2.4 mb)
Supplementary material 1 (PPT 2407 kb)


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Abhishek Rege
    • 1
  • Nitish V. Thakor
    • 1
  • Kevin Rhie
    • 2
  • Arvind P. Pathak
    • 2
    Email author
  1. 1.Department of Biomedical EngineeringThe Johns Hopkins University School of MedicineBaltimoreUSA
  2. 2.Russell H. Morgan Department of Radiology and Radiological SciencesThe Johns Hopkins University School of MedicineBaltimoreUSA

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