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Aerobiologia

pp 1–12 | Cite as

Extractable lipids from Phleum pratense pollen grains and their modifications by ozone exposure

  • Jinane Farah
  • Marie Choël
  • Patricia de Nadaï
  • Sylvie Gosselin
  • Denis Petitprez
  • Moomem Baroudi
  • Nicolas VisezEmail author
Original Paper
  • 13 Downloads

Abstract

Grass pollen grains are an important source of aeroallergens eliciting respiratory allergic diseases worldwide. In the field of allergology, Phleum pratense pollen is considered as a model for other grass taxa. Upon contact with the aqueous phase of mucosa membranes, lipids are co-delivered from pollen grains with allergens. Lipid molecular species have pro-allergic, pro-inflammatory and immunomodulatory effects on the cells of the allergic immune response. The quantitative analysis of Phleum pratense pollen lipids is missing in the literature. In this work, the total mass of lipids extractable by methylene chloride was determined to be 22 ± 1 µg per mg of Phleum pratense pollen. Eighteen percent of the lipidic mass was quantified and identified by gas chromatographic analysis. Identified lipids included alkanes, alkenes, saturated and unsaturated fatty acids, aldehydes and alcohols. Pollen samples harvested at different times and locations showed a very similar lipidic pattern, both qualitatively and quantitatively. Laboratory ozone exposure of pollen substantially modified its lipidic composition by reactions of ozone with alkenes leading to the production of fatty acids and aldehydes. A deeper knowledge of the lipids released by pollen grains, including pollen from polluted areas, is essential for a better understanding of the chemical environment at the cellular level where allergic reactions take place.

Keywords

Pollen Allergy Lipidomics Timothy grass Air pollution 

Notes

Acknowledgements

NV thanks the University of Lille and the Institut de Recherches Pluridisciplinaires en Sciences de l’Environnement (IRePSE Fed 4129) for financial support. The CaPPA project (Chemical and Physical Properties of the Atmosphere) is funded by the French National Research Agency (ANR) through the PIA (Programme d'Investissement d'Avenir) under contract ANR-11-LABX-005-01. This work is a contribution to the CPER research project CLIMIBIO. The authors thank the French Ministère de l'Enseignement Supérieur et de la Recherche, the Hauts de France Region and the European Funds for Regional Economic Development for their financial support to this work.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Univ. Lille, CNRS, UMR 8522 - PC2A - Physicochimie des Processus de Combustion et de l’AtmosphèreLilleFrance
  2. 2.Faculty of Public Health (FSP III), Water & Environment Science LaboratoryLebanese UniversityTripoliLebanon
  3. 3.Univ. Lille, CNRS, UMR 8516 - LASIR - Laboratoire de Spectrochimie Infrarouge et RamanLilleFrance
  4. 4.Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Center for Infection and Immunity of LilleLilleFrance
  5. 5.Association Pour La Prévention de La Pollution AtmosphériqueLoosFrance

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