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Aerobiologia

pp 1–10 | Cite as

The abundance of urban endotoxins as measured with an impinger-based sampling strategy

  • Serena Moretti
  • Wenke Smets
  • Eline Oerlemans
  • Ronny Blust
  • Sarah Lebeer
Original Paper

Abstract

Endotoxins are components of Gram-negative bacteria with inherently high pro-inflammatory potential. In an urban environment, airborne endotoxins may associate with pollutants such as particulate matter, increasing the severity of the immune response by acting as a natural adjuvant to augment inflammatory respiratory disease development. Here, we present a closer look at outdoor urban endotoxins by applying a microbial-targeted collection strategy. Results from 87 samples distributed throughout the city of Antwerp ranged from 0.45 to 93.71 EU/m3, with a geometric mean of 4.49 EU/m3 and 95% confidence interval of 3.53–5.71 EU/m3. Sample collection was also coupled with the use of a Coulter counter, for which the particle count (2.5–10 μm/m3) showed a significant correlation with endotoxin concentration (R2 = 0.24; p < 0.0001; n = 64). In addition, the analysis of the cultivable bacterial colony-forming units on Reasoner’s 2A agar (expressed CFU/m3) showed to be a good indicator for airborne endotoxins (R2 = 0.57; p < 0.0001; n = 58). Moreover, identification of dominant bacterial colonies on these culture plates gave some indications on potential sources of these urban outdoor bacteria and endotoxins.

Keywords

Endotoxin Urban air quality Particulate matter Lipopolysaccharides Respiratory health 

Notes

Acknowledgements

We acknowledge the valuable help of Karin Van den Bergh (SPHERE, University Antwerp, Belgium) for her assistance with the Coulter counter measurements. This research was financially supported by the University of Antwerp (BOF), EUROSA, and the Fund for Scientific Research in Flanders (KaN research Grant Number 1507114N). Serena Moretti is currently holding a Ph.D. scholarship (FWO aspirant).

Supplementary material

10453_2018_9525_MOESM1_ESM.docx (419 kb)
Supplementary material 1 (DOCX 418 kb)
10453_2018_9525_MOESM2_ESM.xlsx (16 kb)
Supplementary material 2 (XLSX 15 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Environmental Ecology and Applied Microbiology (ENdEMIC), Department of Bioscience EngineeringUniversity of AntwerpAntwerpBelgium
  2. 2.Systemic Physiological and Ecotoxicological Research (SPHERE), Department of BiologyUniversity of AntwerpAntwerpBelgium

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