Aerobiologia

, Volume 27, Issue 4, pp 277–290 | Cite as

Characterization of halotolerant and oligotrophic bacterial communities in Asian desert dust (KOSA) bioaerosol accumulated in layers of snow on Mount Tateyama, Central Japan

  • Teruya Maki
  • Kazuma Aoki
  • Fumihisa Kobayashi
  • Makiko Kakikawa
  • Yutaka Tobo
  • Atsushi Matsuki
  • Hiroshi Hasegawa
  • Yasunobu Iwasaka
Original Paper

Abstract

Microbial particles transported by Asian desert dust (KOSA) possibly impact ecosystems and human health in downwind environments and are commonly called “bioaerosols.” The microbial communities associated with KOSA mineral particles (KOSA bioaerosol), which were collected from the snow cover on Mt. Tateyama, were investigated by means of a culture-amendment technique combined with denaturing gradient gel electrophoresis (DGGE) analysis using 16S rRNA genes. After the stratigraphy of the snow layer formed on the walls of a snow pit on Mt. Tateyama, samples were collected from 2 layers, which included KOSA particles and one which did not. The snow samples with KOSA particles indicated microbial growth in the 100 and 10−1 dilution media and in the medium with NaCl below 10%, while the snow sample without KOSA particles showed no microbial growth in the culture media. The PCR–DGGE analysis revealed that the bacterial compositions in the snow samples including KOSA mineral particles were mainly composed of the members of the phyla Actinobacteria, Firmicutus, and Proteobacteria. In particular, the 2 phylotypes appeared in the microbial cultures were similar to the members of the B. subtilis group, which has been detected in bioaerosol samples collected from the atmosphere over KOSA arrival (Suzu City) and source (Dunhuang City) regions. Presumably, halotolerant and oligotrophic bacterial communities are associated with the KOSA particles that descend to the snow cover on Mt. Tateyama.

Keywords

KOSA Snow cover Bioaerosol Bacteria 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Teruya Maki
    • 1
  • Kazuma Aoki
    • 2
  • Fumihisa Kobayashi
    • 1
  • Makiko Kakikawa
    • 1
  • Yutaka Tobo
    • 3
    • 4
  • Atsushi Matsuki
    • 4
  • Hiroshi Hasegawa
    • 1
  • Yasunobu Iwasaka
    • 4
  1. 1.College of Science and EngineeringKanazawa UniversityKanazawaJapan
  2. 2.Department of Earth Sciences, Faculty of ScienceUniversity of ToyamaToyamaJapan
  3. 3.Department of Atmospheric ScienceColorado State UniversityCollinsUSA
  4. 4.Frontier Science OrganizationKanazawa UniversityKanazawaJapan

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