, Volume 21, Issue 3–4, pp 233–239

Comparison of the Rotorod to other air samplers for the determination of Ambrosia artemisiifolia pollen concentrations conducted in the Environmental Exposure Unit

  • Matthew J. Heffer
  • Jodan D. Ratz
  • J. David. Miller
  • James H. Day

DOI: 10.1007/s10453-005-9007-6

Cite this article as:
Heffer, M.J., Ratz, J.D., Miller, J.D. et al. Aerobiologia (2005) 21: 233. doi:10.1007/s10453-005-9007-6


The Environmental Exposure Unit (EEU) is a 924 m3 facility (Kingston General Hospital, Ontario) in which uniform concentrations of various pollens in HEPA-filtered air at known rates of laminar airflow can be maintained. This facility provided a unique opportunity to compare several air samplers without the environmental variation inherent in outdoor comparisons. The purpose of this study was to conduct a quantitative comparison of pollen measurements using the Rotorod, Burkard™ Personal Volumetric Air Sampler, Air-O-Cell™ and a 37 mm open-faced filter cassette with a microporous filter in the EEU. Pollen samples were taken during clinical trials being conducted in the Unit. Raw pollen counts/m3 obtained using the different methods were corrected using published particle collection efficiencies for the particle size (∼ ∼20 μm) and airflow. Data were analyzed by ANOVA/Tukey HSD. No statistically significant differences were found between pollen concentrations determined by Rotorod, Air-O-Cell and filter cassette. Pollen levels determined by the Burkard were up to 2 times higher than the other sampling methods. Relative standard deviations were similar for the Rotorod, Burkard, and filter cassette and higher for the Air-O-Cell. This study demonstrated that, under our conditions, the Rotorod sampler provides consistent and reliable measurements of ragweed pollen concentrations.


Air-O-Cell air sampler Ambrosia artemisiifolia Burkard Environmental Exposure Unit filter cassette pollen Rotorod 





Burkard™ Personal Volumetric Air Sampler


Environmental Exposure Unit

filter cassette

37-mm open-faced filter cassette with a microporous filter

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Matthew J. Heffer
    • 1
  • Jodan D. Ratz
    • 1
  • J. David. Miller
    • 2
  • James H. Day
    • 1
  1. 1.Division of Allergy and ImmunologyKingston General HospitalKingstonCanada
  2. 2.Department of Chemistry, Ottawa-Carleton Institute of ChemistryCollege of Natural Sciences, Carleton UniversityOttawaCanada

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