Abstract
The effects of acute solar radiation stress on photosynthetic efficiency in freshwater unialgal cultures representing three phytoplankton pigment groups were measured by pulse amplitude modulated fluorometry (Walz Phyto-PAM) and compared to previous observations on field populations. Ultraviolet radiation (UVR) (UV-B and UV-A) induced a loss of photochemical quantum efficiency (Fv/Fm) in all 13 taxa examined in culture, while effects of photosynthetically active radiation (PAR) were smaller and often insignificant. Cyanobacteria were the most sensitive to PAR and UVR stress, chlorophytes the least and chromophytes intermediate but variable. The kinetics of maximal (Fm) and minimal (F0) fluorescence responses suggested uncoupling of antenna pigments from reaction centers (decreased Fm) persistent after dark adaptation was a common response, in particular for chromophytes, while the extent of impairment from damaged reaction centers (increased F0) was more variable. Changes in Fv/Fm with irradiance exposure were well described by the Kok model of photoinhibition and indicated that damage, rather than recovery, processes were predictive of acute cumulative inhibition. Field populations of cyanobacteria and chromophytes tended to greater tolerance and lower damage rates than laboratory strains. The results for cultures under standardized conditions supported field results in showing cyanobacteria more sensitive to acute UVR exposure than eukaryotic algae, and thus lacking any innate resistance of photosystem II to sunlight stress that might help explain their success in surface bloom formation.
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Abbreviations
- F 0 :
-
Minimal fluorescence
- F v :
-
Variable fluorescence
- F m :
-
Maximal fluorescence
- F v/F m :
-
Maximum quantum yield of photochemistry
- Chl a, b, c :
-
Chlorophyll a, b and c, respectively
- NPQ:
-
Non-photochemical quenching
- P:
-
PAR-only experimental light treatment
- PA:
-
PAR + UV-A experimental light treatment
- PAB:
-
PAR + UV-A + UV-B experimental light treatment
- PAR:
-
Photosynthetically active radiation
- PAM:
-
Pulse amplitude modulation
- PC:
-
Phycocyanin
- PE:
-
Phycoerythrin
- PSII:
-
Photosystem II
- ROS:
-
Reactive oxygen species
- UVR:
-
Ultraviolet radiation
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The main funding for this research was from an NSERC Discovery Grant (R. Smith), with important additional support from Environment and Climate Change Canada (S. Watson).
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Beecraft, L., Watson, S.B. & Smith, R.E.H. Innate resistance of PSII efficiency to sunlight stress is not an advantage for cyanobacteria compared to eukaryotic phytoplankton. Aquat Ecol 53, 347–364 (2019). https://doi.org/10.1007/s10452-019-09694-4
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DOI: https://doi.org/10.1007/s10452-019-09694-4