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Aquatic Ecology

, Volume 51, Issue 1, pp 145–158 | Cite as

Influence of the colonizing substrate on diatom assemblages and implications for bioassessment: a mesocosm experiment

  • C. L. Elias
  • R. J. M. Rocha
  • M. J. Feio
  • E. Figueira
  • S. F. P. Almeida
Article

Abstract

Although diatoms are important bioindicators of ecological quality, their ecological traits are still not well understood. A major issue is that of substrate preferences, which may result in differences in production, and assemblage structure and composition, and which should therefore be taken into account for ecological quality assessment studies. Thus, in this work, the periphyton grown on sand and ceramic tiles in indoor controlled channels were compared to understand whether substrate differences lead to differences in: periphyton production (chlorophyll-a), chlorophyll-b and c concentrations, diatom assemblages (diversity-Shannon-Wiener, cell density, taxonomic composition, trait proportions), and ecological quality assessments (IPS-‘Indice de Polluosensibilité Spécifique’). A combined inoculum of periphyton from four Portuguese streams was introduced to the running channels (six sand and six tile) and left to colonize for 35 days. Epilithic (tiles) and epipsammic (sand) assemblages were sampled at days 14 and 35. We verified that there were no differences in chlorophyll-a concentration over time and between substrates. On both sampling occasions, the epipsammic assemblages had higher concentration of chlorophyll-c and diatom density but without significant differences over time in each substrate. The taxonomic composition was different between substrates and over time. However, these differences were not reflected in ecological quality assessment. The diversity was also similar between substrates in both sampling occasions, but it was higher at day 14. Mobile and stalked species were more abundant over the entire study and differed significantly between substrates, with the epipsammic assemblages presenting higher abundances of both traits. We concluded that the colonizing substrate influences diatom assemblages but not the ecological quality assessment.

Keywords

Diatoms Chlorophyll Traits Mesocosm Ecological quality assessment Freshwater 

Notes

Acknowledgements

This study was possible due to the financial support of the FOUNDATION FOR SCIENCE AND TECHNOLOGY (Portugal) through the Ph.D. scholarship SFRH/BD/68973/2010 of the first author and through the strategic project UID/MAR/04292/2013 granted to MARE and UID/GEO/04035/2013 granted to GeoBioTec. We thank GeoBioTec Research Centre and Biology Department, University of Aveiro. We thank to the Engineer Acácio Pascoal from the company Gres Panaria, Portugal S.A.—LOVE TILES division for the offer of the ceramic tiles and to the company Water Technologies for all the technical and equipment support.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • C. L. Elias
    • 1
    • 2
  • R. J. M. Rocha
    • 3
  • M. J. Feio
    • 2
  • E. Figueira
    • 3
  • S. F. P. Almeida
    • 1
  1. 1.Department of Biology and GeoBioTec – GeoBioSciences, GeoTechnologies and GeoEngineering Research CentreUniversity of AveiroAveiroPortugal
  2. 2.MARE-Marine and Environmental Sciences Centre, Department of Life SciencesUniversity of CoimbraCoimbraPortugal
  3. 3.Department of Biology and CESAM – Centre for Environmental and Marine StudiesUniversity of AveiroAveiroPortugal

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