, Volume 16, Issue 4–5, pp 259–269 | Cite as

Irreversible adsorption of latex particles on fibrinogen covered mica

  • Z. AdamczykEmail author
  • M. Nattich
  • M. Wasilewska
Open Access


Physicochemical properties of bovine plasma fibrinogen (Fb) in electrolyte solutions were characterized. These comprised the diffusion coefficient (hydrodynamic radius), determined by the DLS method, electrophoretic mobility and the isoelectric point. The hydrodynamic radius of Fb was 12 nm for pH<5. The number of uncompensated (electrokinetic) charges on the protein N c was calculated from the electrophoretic mobility data. It was found that for pH<5.8 the electrokinetic charge was positive, independently of the ionic strength and negative for pH>5.8. For pH=3.5 the value of N c , was 26 for 10−3 M. Similar electrokinetic measurements were performed for the mica substrate using the streaming potential cell. It was shown that for pH=3.5 and 10−3 M, the zeta potential of mica remained negative (−50 mV). This promoted an irreversible, electrostatically driven adsorption of Fb, which was confirmed in experiments carried out under diffusion-controlled transport. The surface concentration of Fb on mica was determined directly by AFM counting. By adjusting the time of adsorption, Fb monolayers of desired coverage were produced. Independently, the presence of Fb on mica was determined quantitatively by the colloid enhancement method, in which negatively charged latex particles were used, having the diameter of 800 nm. It was found that for Fb coverage below 0.05 the method was more sensitive than other indirect methods. The experimental data obtained in latex deposition experiments were adequately interpreted in terms of the random site model used previously for polyelectrolytes. It was shown that adsorption sites consisted of a cluster of two Fb molecules. It was concluded that the colloid enhancement method can be successfully used for detecting the presence of proteins at solid substrates and to determine the uniformity of monolayers in the nanoscale.


Fibrinogen monolayers on mica Colloid enhancement of protein layers Adsorption of latex on fibrinogen Irreversible adsorption of colloid particles 


  1. Adamczyk, Z.: J. Colloid Interface Sci. 220, 477 (2000) CrossRefGoogle Scholar
  2. Adamczyk, Z.: Particles at Interfaces: Interactions, Deposition, Structure. Academic Press/Elsevier, New York (2006) Google Scholar
  3. Adamczyk, Z., Zembala, M., Siwek, B., Warszyński, P.: J. Colloid Interface Sci. 140, 123–124 (1990) CrossRefGoogle Scholar
  4. Adamczyk, Z., Senger, B., Voegel, J.C., Schaaf, P.: J. Chem. Phys. 110, 3118 (1999) CrossRefGoogle Scholar
  5. Adamczyk, Z., Warszyński, P., Zembala, M.: Bull. Pol. Acad. Chem. 47, 239–258 (1999) Google Scholar
  6. Adamczyk, Z., Zembala, M., Michna, A.: J. Colloid Interface Sci. 303, 353–364 (2006) CrossRefGoogle Scholar
  7. Adamczyk, Z., Michna, A., Szaraniec, M., Bratek, A., Barbasz, J.: J. Colloid Interface Sci. 313, 86–96 (2007) CrossRefGoogle Scholar
  8. Adamczyk, Z., Sadlej, K., Wajnryb, E., Nattich, M., Ekiel-Jeżewska, M.L., Bławzdziewicz, J.: Adv. Colloid Interface Sci. 153, 1–29 (2010) CrossRefGoogle Scholar
  9. Adamczyk, Z., Barbasz, J., Cieśla, M.: Langmuir (2010, in press) Google Scholar
  10. Adamczyk, Z., Zaucha, M., Zembala, M.: Langmuir 26, 9368 (2010) CrossRefGoogle Scholar
  11. Agnihotri, A., Siedlecki, C.A.: Langmuir 20, 8846 (2004) CrossRefGoogle Scholar
  12. Buijs, J., van den Berg, P.A.W., Lichtenbelt, J.W.Th., Norde, W., Lyklema, J.: J. Colloid Interface Sci. 178, 594–605 (1996) CrossRefGoogle Scholar
  13. Buijs, J., White, D.D., Norde, W.: Colloids Surf. B, Biointerfaces 8, 239–249 (1997) CrossRefGoogle Scholar
  14. Choi, K.H., Friedt, J.M., Frederix, F., Campitelli, A., Borghs, G.: Appl. Phys. Lett. 81, 1335–1337 (2002) CrossRefGoogle Scholar
  15. Elgersma, A.V., Zsom, R.L.J., Lyklema, J., Norde, W.: Colloid Surf. A 65, 17–28 (1992) CrossRefGoogle Scholar
  16. Etheve, J., Dejardin, P.: Langmuir 18, 1777–1785 (2002) CrossRefGoogle Scholar
  17. Hall, C.E., Slayter, H.S.J.: Biophys. Biochem. Cytol. 5, 11 (1959) CrossRefGoogle Scholar
  18. Hayes, R.A., Biehmer, M.R., Fokkink, L.G.J.: Langmuir 15, 2865–2870 (1999) CrossRefGoogle Scholar
  19. Hinrichsen, E.L., Feder, J., Jossang, T.: J. Stat. Phys. 44, 793 (1986) CrossRefGoogle Scholar
  20. Jin, X., Wang, N.H.L., Tarjus, G., Talbot, J.: J. Phys. Chem. 97, 4256–4258 (1993) CrossRefGoogle Scholar
  21. Marchin, K.L., Berrie, C.L.: Langmuir 19, 9883–9888 (2003) CrossRefGoogle Scholar
  22. Melmsten, M.: J. Colloid Interface Sci. 166, 333–342 (1994) CrossRefGoogle Scholar
  23. Norde, W., Rouwendal, E.: J. Colloid Interface Sci. 139, 169–176 (1990) CrossRefGoogle Scholar
  24. Ortega-Vinuesa, J.L., Tengvall, P., Lundstrom, I.: Thin Solid Films 325, 257 (1998) CrossRefGoogle Scholar
  25. Ramsden, J.J.: Q. Rev. Bipohys. 27, 41–105 (1993) CrossRefGoogle Scholar
  26. Reisch, A., Voegel, J.C., Gonthier, E., Decher, G., Senger, B., Schaaf, P., Mésini, P.J.: Langmuir 25, 3610–3617 (2009) CrossRefGoogle Scholar
  27. Scales, P.J., Grieser, F., Healy, T.W.: Langmuir 6, 582–589 (1990) CrossRefGoogle Scholar
  28. Scales, P.J., Grieser, F., Healy, T.W.: Langmuir 8, 965–974 (1992) CrossRefGoogle Scholar
  29. Schaaf, P., Talbot, J.: J. Chem. Phys. 91, 4401 (1989) CrossRefGoogle Scholar
  30. Sit, P.S., Marchant, R.E.: Thromb. Haemost. 82, 1053–1060 (1999) Google Scholar
  31. Toscano, A., Santore, M.M.: Langmuir 22, 2588 (2006) CrossRefGoogle Scholar
  32. van Wagenen, R.A., Andrade, J.D.: J. Colloid Interface Sci. 76, 305–314 (1980) CrossRefGoogle Scholar
  33. Vasina, E.N., Dejardin, P.: Langmuir 20, 8699–8706 (2004) CrossRefGoogle Scholar
  34. Veklich, Y.I., Gorkun, O.V., Medved, L.V., Nieuwenhuizen, W., Weisel, J.W.: J. Biol. Chem. 268, 13577–13585 (1993) Google Scholar
  35. Wasilewska, M., Adamczyk, Z., Jachimska, B.: Langmuir 25, 369 (2009) CrossRefGoogle Scholar
  36. Wertz, Ch.F., Santore, M.M.: Langmuir 17, 3006–3010 (2001) CrossRefGoogle Scholar
  37. Yoon, J.Y., Park, H.Y., Kim, J.H., Kim, W.S.: J. Colloid Interface Sci. 177, 613–620 (1996) CrossRefGoogle Scholar
  38. Zembala, M., Adamczyk, Z.: Langmuir 16, 1593–1601 (2000) CrossRefGoogle Scholar
  39. Zembala, M., Dejardin, P.: Colloids Surf. B 3, 119–129 (1994) CrossRefGoogle Scholar
  40. Zembala, M., Voegel, J.C., Schaaf, P.: Langmuir 14, 2167–2173 (1998) CrossRefGoogle Scholar
  41. Zembala, M., Adamczyk, Z., Warszyński, P.: Colloid Surf. A 222, 329–339 (2003) CrossRefGoogle Scholar

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© The Author(s) 2010

Authors and Affiliations

  1. 1.Institute of Catalysis and Surface ChemistryPolish Academy of SciencesCracowPoland

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