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Adsorption

, Volume 11, Issue 5–6, pp 595–602 | Cite as

Synthesis and Characterization of Silica Gel Particles Functionalized with Bioactive Materials

  • M. A. RodriguesEmail author
  • M. P. Bemquerer
  • D. B. Tada
  • E. L. Bastos
  • M. S. Baptista
  • M. J. Politi
Article

Abstract

Bioactive materials (having an amino acid, Ac-Tryptophan, A or a peptide, Ac-Trp-Ala-Ala, B) were anchored onto silica particles. A photoactive linker (N,N′-bis(2-phosphonoethyl)-1,4,5,8-naphthalenediimide (DPN)) was initially attached to the particles and next the amino acids were bound by using both Zr/P chemistry and 1,3-diisopropylcarbodiimide/1-hydroxybenzotriazole coupling. In A derivative extensive complexation of Trp with DPN was observed. Photolysis studies showed the presence of excited state reactions on the silica particles, moreover the radical species (DPN.−, TrpH.+, Trp.) remained alive ∼100 times longer on the particle surface than in solution. These studies show that the formation rate of these radicals is a function of the supramolecular structures of the peptide and of the amino acid derivative on the silica particle.

Keywords

Peptide Thin Film Excited State Photolysis Formation Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • M. A. Rodrigues
    • 1
    Email author
  • M. P. Bemquerer
    • 1
  • D. B. Tada
    • 2
  • E. L. Bastos
    • 2
  • M. S. Baptista
    • 2
  • M. J. Politi
    • 2
  1. 1.Departamento de Bioquímica e Imunologia, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrasil
  2. 2.Departamento de Bioquímica, Instituto de QuímicaUniversidade de São PauloSão PauloBrasil

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