Annals of Biomedical Engineering

, Volume 47, Issue 11, pp 2308–2321 | Cite as

Effects of a Bioactive SPPEPS Peptide on Chondrogenic Differentiation of Mesenchymal Stem Cells

  • Salma Mahzoon
  • Jakob M. Townsend
  • Thi N. Lam
  • Virginie Sjoelund
  • Michael S. DetamoreEmail author


A synthetic ‘chondroinductive’ biomaterial that could induce chondrogenesis without the need for growth factors, extracellular matrix, or pre-seeded cells could revolutionize orthopedic regenerative medicine. The objective of the current study was thus to introduce a synthetic SPPEPS peptide and evaluate its ability to induce chondrogenic differentiation. In the current study, dissolving a synthetic chondroinductive peptide candidate (100 ng/mL SPPEPS) in the culture medium of rat bone marrow-derived mesenchymal stem cells (rBMSCs) elevated collagen type II gene expression compared to the negative control (no growth factor or peptide in the cell culture medium) after 3 days. In addition, proteomic analyses indicated similarities in pathways and protein profiles between the positive control (10 ng/mL TGF-β3) and peptide group (100 ng/mL SPPEPS), affirming the potential of the peptide for chondroinductivity. Incorporating the SPPEPS peptide in combination with the RGD peptide in pentenoate-functionalized hyaluronic acid (PHA) hydrogels elevated the collagen type II gene expression of the rBMSCs cultured on top of the hydrogels compared to using either peptide alone. The evidence suggests that SPPEPS may be a chondroinductive peptide, which may be enhanced in combination with an adhesion peptide.


Chondroinductive peptide Biomaterial Hydrogel Proteomics 



The authors would like to thank Emi Kiyotake for assistance with preparing the manuscript. The authors acknowledge Dr. Susan Nimmo at the University of Oklahoma Magnetic Resonance Facility for help with conducting NMR. The authors acknowledge a potential conflict of interest as the authors (S.M. and M.D.) are actively pursuing intellectual property protection of the SPPEPS peptide.

Supplementary material

10439_2019_2306_MOESM1_ESM.pdf (206 kb)
Supplementary material 1 (PDF 206 kb)


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Copyright information

© Biomedical Engineering Society 2019

Authors and Affiliations

  1. 1.School of Aerospace and Mechanical EngineeringUniversity of OklahomaNormanUSA
  2. 2.Stephenson School of Biomedical EngineeringUniversity of OklahomaNormanUSA
  3. 3.Department of Microbiology and Plant BiologyUniversity of OklahomaNormanUSA
  4. 4.Department of Cell BiologyUniversity of Oklahoma Health Science CenterOklahoma CityUSA

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