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Annals of Biomedical Engineering

, Volume 47, Issue 5, pp 1250–1264 | Cite as

Non-Destructive Reflectance Mapping of Collagen Fiber Alignment in Heart Valve Leaflets

  • Will Goth
  • Sam Potter
  • Alicia C. B. Allen
  • Janet Zoldan
  • Michael S. Sacks
  • James W. TunnellEmail author
Article

Abstract

Collagen fibers are the primary structural elements that define many soft-tissue structure and mechanical function relationships, so that quantification of collagen organization is essential to many disciplines. Current tissue-level collagen fiber imaging techniques remain limited in their ability to quantify fiber organization at macroscopic spatial scales and multiple time points, especially in a non-contacting manner, requiring no modifications to the tissue, and in near real-time. Our group has previously developed polarized spatial frequency domain imaging (pSFDI), a reflectance imaging technique that rapidly and non-destructively quantifies planar collagen fiber orientation in superficial layers of soft tissues over large fields-of-view. In this current work, we extend the light scattering models and image processing techniques to extract a critical measure of the degree of collagen fiber alignment, the normalized orientation index (NOI), directly from pSFDI data. Electrospun fiber samples with architectures similar to many collagenous soft tissues and known NOI were used for validation. An inverse model was then used to extract NOI from pSFDI measurements of aortic heart valve leaflets and clearly demonstrated changes in degree of fiber alignment between opposing sides of the sample. These results show that our model was capable of extracting absolute measures of degree of fiber alignment in superficial layers of heart valve leaflets with only general a priori knowledge of fiber properties, providing a novel approach to rapid, non-destructive study of microstructure in heart valve leaflets using a reflectance geometry.

Keywords

Polarized light imaging Structured illumination Collagen fiber structure Cylindrical scattering Optical sectioning Wide-field reflectance imaging 

Notes

Acknowledgments

This work was supported by funding from the National Heart, Lung, and Blood Institute of the National Institutes of Health (awards RO1-HL108330 and RO1-HL129077), the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health (Award T32-EB007505), and the Cancer Prevention and Research Institute of Texas (Award RP-130702). The authors would also like to thank Mason Dana for his contributions to data collection and instrumentation troubleshooting, and acknowledge the Microscopy and Imaging Facility of the Institute for Cellular and Molecular Biology at The University of Texas at Austin for use of their electron microscope facilities. There are no conflicts of interest from financial or other commercial benefits related to the development of this manuscript.

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Copyright information

© Biomedical Engineering Society 2019

Authors and Affiliations

  1. 1.Department of Biomedical EngineeringThe University of Texas at AustinAustinUSA
  2. 2.Department of Mechanical EngineeringThe University of Texas at AustinAustinUSA
  3. 3.James T. Willerson Center for Cardiovascular Modeling and Simulation, Institute for Computational Engineering and Sciences, Department of Biomedical EngineeringThe University of Texas at AustinAustinUSA

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