Annals of Biomedical Engineering

, Volume 43, Issue 9, pp 2220–2230 | Cite as

Synergistic Impact of Nicotine and Shear Stress Induces Cytoskeleton Collapse and Apoptosis in Endothelial Cells

  • Yu-Hsiang Lee
  • Ruei-Siang Chen
  • Nen-Chung Chang
  • Kueir-Rarn Lee
  • Chien-Tsai Huang
  • Yu-Ching Huang
  • Feng-Ming Ho
Article

Abstract

Nicotine is the major component in cigarette smoke and has been recognized as a risk factor for various cardiovascular diseases such as atherosclerosis. However, the definite pathogenesis of nicotine-mediated endothelial dysfunction remains unclear because hemodynamic factor in most of prior in vitro studies was excluded. To understand how nicotine affects endothelium in the dynamic environment, human umbilical vein endothelial cells were treated by different laminar shear stresses (LSS; 0, 6, 8, and 12 dynes cm−2) with and without 10−4 M nicotine for 12 h in a parallel plate flow system, following detections of cellular morphology and apoptotic level. Our results showed that cells sheared by 12 dynes cm−2 LSS with nicotine excessively elongated and aligned with the flow direction, and exhibited significant apoptosis as compared to the groups with nicotine or LSS alone. We reasoned that the irregular morphological rearrangement and elevated apoptosis were resulted from the interruption of mechanostasis due to cytoskeletal collapse. Furthermore, all the impaired responses can be rescued by treatment with free radical scavenger ascorbic acid (10−4 M), indicating oxidative stress was likely mediated with the impairments. In summary, our findings demonstrated an essential role of LSS in nicotine-mediated endothelial injury occurring in the physiological environment.

Keywords

Nicotine Laminar shear stress Morphological reorganization Cytoskeleton disintegration Apoptosis 

Supplementary material

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Supplementary material 1 (PDF 3899 kb)

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Copyright information

© Biomedical Engineering Society 2015

Authors and Affiliations

  • Yu-Hsiang Lee
    • 1
  • Ruei-Siang Chen
    • 1
  • Nen-Chung Chang
    • 2
  • Kueir-Rarn Lee
    • 3
  • Chien-Tsai Huang
    • 4
  • Yu-Ching Huang
    • 5
  • Feng-Ming Ho
    • 2
    • 3
    • 4
  1. 1.Graduate Institute of Biomedical EngineeringNational Central UniversityTaoyuan CountyTaiwan, ROC
  2. 2.Department of Internal Medicine, School of MedicineTaipei Medical UniversityTaipeiTaiwan, ROC
  3. 3.R&D Center for Membrane Technology, Department of Chemical EngineeringChung Yuan Christian UniversityTaoyuan CountyTaiwan, ROC
  4. 4.Department of Internal MedicineTao-Yuan General Hospital, Ministry of Health and WelfareTaoyuan CountyTaiwan, ROC
  5. 5.Department of NeurologyTao-Yuan General Hospital, Ministry of Health and WelfareTaoyuan CountyTaiwan, ROC

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