Annals of Biomedical Engineering

, Volume 43, Issue 4, pp 1003–1013 | Cite as

Tissue-Engineered Tracheal Reconstruction Using Mesenchymal Stem Cells Seeded on a Porcine Cartilage Powder Scaffold

  • Yoo Seob Shin
  • Jae Won Choi
  • Ju-Kyeong Park
  • Yoo Suk Kim
  • Soon Sim Yang
  • Byoung-Hyun Min
  • Chul-Ho KimEmail author


Tissue engineering using a biocompatible scaffold with various cells might be a solution for tracheal reconstruction. We investigated the plausibility of using mesenchymal stem cells (MSCs) seeded on a porcine cartilage powder (PCP) scaffold for tracheal defect repair. PCP made with minced and decellularized porcine articular cartilage was molded into a 5 × 12 mm (height × diameter) scaffold. MSCs from young rabbit bone marrow were expanded and cultured with the PCP scaffold. After 7 weeks culture, the tracheal implants were transplanted on a 5 × 10 mm tracheal defect in six rabbits. 6 and 10 weeks postoperatively, the implanted area was evaluated. None of the six rabbits showed any sign of respiratory distress. Endoscopic examination revealed that respiratory epithelium completely covered the regenerated trachea and there were no signs of collapse or blockage. A patent luminal contour of the trachea was observed on the computed tomography scan in all six rabbits and the reconstructed areas were not narrow compared to normal adjacent trachea. Histologic examination showed that neo-cartilage was successfully produced with minimal inflammation or granulation tissue. Ciliary beating frequency of the regenerated epithelium was not significantly different from the normal adjacent mucosa. MSCs cultured with a PCP scaffold successfully restored not only the shape but also the function of the trachea without any graft rejection.


Tracheal regeneration Porcine cartilage powder Tissue engineering Mesenchymal stem cell 



This work was supported by 2013 Ajou University Translational Research Center Fund (Seed type).


The authors indicate no financial disclosures.

Conflict of interest



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Copyright information

© Biomedical Engineering Society 2014

Authors and Affiliations

  • Yoo Seob Shin
    • 1
  • Jae Won Choi
    • 2
  • Ju-Kyeong Park
    • 1
    • 2
  • Yoo Suk Kim
    • 1
  • Soon Sim Yang
    • 2
    • 4
  • Byoung-Hyun Min
    • 2
    • 3
    • 4
  • Chul-Ho Kim
    • 1
    • 2
    Email author
  1. 1.Department of OtolaryngologyAjou UniversitySuwonRepublic of Korea
  2. 2.Department of Molecular Science and TechnologyAjou UniversitySuwonRepublic of Korea
  3. 3.Department of Orthopedic Surgery, School of MedicineAjou UniversitySuwonRepublic of Korea
  4. 4.Cell Therapy CenterAjou University Medical CenterSuwonRepublic of Korea

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